Luo Yu-qin, Qian Yu-li, Lu Huan-ming, Xu Chen-ming, Jin Fan
Department of Clinical Laboratory, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310006 PR China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2009 Feb;26(1):54-6. doi: 10.3760/cma.j.issn.1003-9406.2009.01.012.
To analyze the sex chromosome meiotic segregation in inv(Y) patients by fluorescence in situ hybridization (FISH).
Conventional cytogenetic procedures (GTG and CBG banding) and FISH were performed on metaphase chromosome. Three-color FISH was performed on sperm samples using a probe mixture containing CEPX, Tel Xp/Yp and Tel Xq/Yq to investigate the sex chromosome segregation of five inv(Y) (p11.1q11.2) carriers. A healthy man with normal semen parameters was used as control.
There was no statistical difference in the abnormal sex chromosome number and recombination frequencies in each spermatozoon from the patient in comparison with that in the control.
There was no apparent sex chromosome abnormality in the sperm of the inv(Y) (p11.1q11.2) carriers. Sperm-FISH allows further understanding of the sex chromosome segregation pattern and an accurate genetic counseling.
通过荧光原位杂交（FISH）分析inv（Y）患者的性染色体减数分裂分离情况。
对中期染色体进行常规细胞遗传学检查（GTG和CBG显带）及FISH。使用包含CEP X、Tel Xp/Yp和Tel Xq/Yq的探针混合物对5例inv（Y）（p11.1q11.2）携带者的精子样本进行三色FISH，以研究性染色体分离情况。选取一名精液参数正常的健康男性作为对照。
与对照组相比，患者每个精子中性染色体数目异常及重组频率无统计学差异。
inv（Y）（p11.1q11.2）携带者的精子中无明显性染色体异常。精子FISH有助于进一步了解性染色体分离模式并进行准确的遗传咨询。
