[自发性高血压大鼠海绵体中Rho激酶与血红素加氧酶的表达]

[Expression of Rho-kinase and heme oxygenase in the corpus cavernosum of spontaneous hypertensive rats].

作者信息

Zhu Ping-Yu, Jiang Rui, Deng Qing-Fu, Wang Xiao-Ran

机构信息

Department of Urology, the Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan 646000, China.

出版信息

Zhonghua Nan Ke Xue. 2008 Mar;14(3):215-9.

PMID:18488332

Abstract

OBJECTIVE

To investigate the expression of Rho-kinase and heme oxygenase and the interaction among NOS/NO, HO/CO and RhoA/Rho-kinase in the corpus cavernosum of spontaneous hypertensive rats (SHRs).

METHODS

A series of electric stimuli were applied to the corpus cavernosum nerves of 7 SHRs, the changes of ICP/MAP observed continuously and the expressions of ROCK2, HO-2 and eNOS analyzed by Western blot and immunohistochemistry. Another 7 WKY rats were taken as controls.

RESULTS

Compared with the controls, ICP/MAP did not rise obviously (P < 0.05), HO-2 dropped sharply (P = 0.006) and the expression of the ROCK2 protein was elevated markedly (P = 0.017) in the SHRs. Immunohistochemistry showed HO-2 to be distributed in the smooth muscle cells and nervous cells of the corpus cavernosum, and eNOS mainly in the vascular endothelial cells. The expressions of HO-2 and eNOS were obviously reduced in the SHRs.

CONCLUSION

NOS/NO, HO/CO and RhoA/Rho-kinase are related with erectile dysfunction in SHRs and may interact on one another.

摘要

目的

研究自发性高血压大鼠（SHRs）阴茎海绵体内Rho激酶和血红素加氧酶的表达以及一氧化氮合酶/一氧化氮（NOS/NO）、血红素加氧酶/一氧化碳（HO/CO）和RhoA/Rho激酶之间的相互作用。

方法

对7只SHRs的阴茎海绵体神经施加一系列电刺激，持续观察阴茎海绵体内压/平均动脉压（ICP/MAP）的变化，并通过蛋白质免疫印迹法和免疫组织化学法分析Rho激酶2（ROCK2）、血红素加氧酶-2（HO-2）和内皮型一氧化氮合酶（eNOS）的表达。另取7只WKY大鼠作为对照。

结果

与对照组相比，SHRs的ICP/MAP无明显升高（P<0.05），HO-2急剧下降（P = 0.006），ROCK2蛋白表达显著升高（P = 0.017）。免疫组织化学显示，HO-2分布于阴茎海绵体的平滑肌细胞和神经细胞中，eNOS主要分布于血管内皮细胞中。SHRs中HO-2和eNOS的表达明显降低。