Deng Qing-fu, Jiang Rui, Zhu Ping-yu, Wang Xiao-ran
Department of Urology, the Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan 646000, China.
Zhonghua Nan Ke Xue. 2008 Jun;14(6):503-6.
To determine the expressions of HO-2 and eNOS in the corpus cavernosum of castrated rats and investigate the correlation of HO-2 and eNOS with erectile dysfunction.
Forty SD rats of 10 weeks were equally divided into 3 castrated groups (4, 8 and 12 weeks respectively after removal of bilateral testes) and a sham operation control group. All the rats were detected for serum testosterone (T), intracavernosal pressure (ICP) and mean arterial blood pressure (MAP) 2, 8 and 12 weeks after the surgery, the content of HO-2 analyzed by Western blot and the expressions of eNOS and HO-2 determined by immunohistochemistry.
Testosterone level and ICP/MAP ratio were markedly decreased after the castration (P < 0.05). The HO-2 expression was detected in the corpus cavernosum of all but the 12-week castrated rats, and the area under the curve was (341.50 +/- 99.70) in the 4-week castrated group, significantly less than in the 8-week (705.00 +/- 152. 74) and the control group (876 +/- 443. 36) (P < 0. 05), with no obvious difference between the latter two. eNOS was found mainly in the vascular endothelial cells of the corpus cavernosum, significantly less in the castrated (123.94 +/- 30. 23) than in the control group (421.21 +/- 125. 12).
Androgens might mediate the penile erectile function through HO-2 and eNOS in the corpus cavernosum of rats.
检测去势大鼠阴茎海绵体内血红素加氧酶-2（HO-2）和内皮型一氧化氮合酶（eNOS）的表达，探讨HO-2、eNOS与勃起功能障碍的相关性。
将40只10周龄的SD大鼠平均分为3个去势组（分别于双侧睾丸切除后4、8和12周）和1个假手术对照组。术后2、8和12周检测所有大鼠的血清睾酮（T）、海绵体内压（ICP）和平均动脉血压（MAP），采用蛋白质免疫印迹法分析HO-2含量，免疫组织化学法检测eNOS和HO-2的表达。
去势后睾酮水平及ICP/MAP比值明显降低（P<0.05）。除12周去势组外，其余各组阴茎海绵体均检测到HO-2表达，4周去势组曲线下面积为（341.50±99.70），明显低于8周去势组（705.00±152.74）和对照组（876±443.36）（P<0.05），后两者差异无统计学意义。eNOS主要分布于阴茎海绵体血管内皮细胞，去势组（123.94±30.23）明显低于对照组（421.21±125.12）。
雄激素可能通过大鼠阴茎海绵体内的HO-2和eNOS介导阴茎勃起功能。
