Abad Z Gloria, Abad Jorge A, Coffey Michael D, Oudemans Peter V, Man in 't Veld Willem A, de Gruyter Hans, Cunnington James, Louws Frank J
United States Department of Agriculture, USDA-APHIS-PPQ-PHP-PSPI, National Identification Service (NIS), Molecular Diagnostics Laboratory (MDL), Bldg. 580, BARC-E, Powder Mill Road, Beltsville, Maryland 20705, USA.
Mycologia. 2008 Jan-Feb;100(1):99-110. doi: 10.3852/mycologia.100.1.99.
A homothallic semipapillate slow growing Phytophthora species associated with root rot of strawberries from greenhouse-grown plants in North Carolina, USA, root rot of roses in the Netherlands, and root rot of raspberry in Knoxfield, Australia, was identified. The main character of this organism is the production of paragynous antheridia with broad attachment to the oogonial wall. The morphology of the pathogen does not match that of any of the more than 85 described Phytophthora species. Phylogenetic analysis based on sequences of the internal transcribed spacer rDNA region (ITS1-5.8S-ITS2) of this taxon and those from other Phytophthora species from GenBank supports the conclusion that this organism is an unreported new species. In the phylogenetic tree with other reported Phytophthora species at the GenBank, the new species is more closely related to others in ITS clade 2 comprising semipapillate taxa including P. botryosa, P. citrophthora, P. colocasiae, P. meadii, P. citricola, P. inflata, P.tropicalis, P. capsici, Phytophthora sp. 'glovera' and P. multivesiculata. The most closely related species is P. multivesiculata isolated from Cymbidium orchid in the Netherlands. In this paper we describe the morphological characteristics and the phylogenetic relationships that support the description of this taxon as a new species Phytophthora bisheria sp. nov.
一种同宗配合、半乳头状、生长缓慢的疫霉属物种被鉴定出来，它与美国北卡罗来纳州温室种植草莓的根腐病、荷兰玫瑰的根腐病以及澳大利亚诺克斯菲尔德树莓的根腐病有关。该生物体的主要特征是产生具宽附着于卵孢子壁的侧生雄器。该病原体的形态与已描述的85种以上疫霉属物种中的任何一种都不匹配。基于该分类单元和来自GenBank的其他疫霉属物种的内部转录间隔区rDNA区域（ITS1-5.8S-ITS2）序列的系统发育分析支持这一结论，即该生物体是一个未报道的新物种。在GenBank中与其他已报道的疫霉属物种构建的系统发育树中，该新物种与ITS进化枝2中的其他物种关系更密切，进化枝2包括半乳头状分类群，如博特里疫霉、柠檬疫霉、芋疫霉、米德疫霉、柑橘疫霉、膨胀疫霉、热带疫霉、辣椒疫霉、疫霉属‘glovera’和多泡疫霉。关系最密切的物种是从荷兰大花蕙兰中分离出 的多泡疫霉。在本文中，我们描述了支持将该分类单元描述为新物种疫霉属比氏疫霉（Phytophthora bisheria sp. nov.）的形态特征和系统发育关系。
