Rotanov M, Grebennikova T V, Burtseva E I, Shevchenko E S
Mol Gen Mikrobiol Virusol. 2008(2):27-32.
The results of molecular analysis of 15 influenza A(H3N2) and 17-A(H1N1) epidemic strains isolated in the Russian Federation in 1995-2007 are described. The analysis on the M2 and neuraminidase influenza A virus genes was performed. The M2 sequences analysis among the remantadin resistant viruses demonstrated the S31N substitution in all strains. Besides S31N substitution, additional mutations were detected in both proteins. Mutations associated with S31N substitution were detected in each virus subtype, which may be considered as new markers for the identification of remantadin-resistant strains. The sequencing of the NA segments from all viruses showed no amino acid substitutions known to cause resistance to neuraminidase inhibitors, which indicates susceptibility to NA inhibitors among the strains.
描述了1995 - 2007年在俄罗斯联邦分离出的15株甲型（H3N2）流感病毒和17株甲型（H1N1）流感流行毒株的分子分析结果。对甲型流感病毒的M2和神经氨酸酶基因进行了分析。对金刚烷胺耐药病毒的M2序列分析表明，所有毒株均有S31N替换。除了S31N替换外，在这两种蛋白中还检测到了其他突变。在每种病毒亚型中都检测到了与S31N替换相关的突变，这可被视为鉴定金刚烷胺耐药毒株的新标记。对所有病毒的NA片段进行测序，未发现已知会导致对神经氨酸酶抑制剂耐药的氨基酸替换，这表明这些毒株对NA抑制剂敏感。
