Yin Xiao-Lin, Hou Tian-Wen, Xu Shu-Bin, Ma Cui-Qing, Yao Zhi-Yan, Li Wei, Wei Lin
Department of Microbiology and Immunology of Hebei Medical University, Shijiazhuang city, Hebei province, People's Republic of China.
Microb Drug Resist. 2008 Jun;14(2):145-50. doi: 10.1089/mdr.2008.0799.
The beta-lactamase (BLA) genes, the genes for aminoglycosides-modifying enzymes (AMEs), disinfectant-sulfanilamide resistance (qacEDelta1-sul1) genes, class 1 integrase (intl1) gene, and the qnr gene associated with plasmid-mediated quinolone resistance were analyzed using PCR and verified by DNA sequencing for 31 clinical isolates of multidrug-resistant Acinetobacter baumannii (MDRAB). The organism typing was performed by pulsed-field gel electrophoresis (PFGE). The positive rate of ADC, TEM, PER, and DHA of BLA genes were 100%, 61.3%, 19.4%, and 3.2%, respectively; however, the genes of SHV, OXA-23 group, OXA-24 group, GES, VIM, IMP, and qnr gene were negative. The positive rate of the genes of AMEs for aac (3)-I, aac (6')-I, ant (3")-I, ant (2")-I, aac (3)-II, and aac (6')-II were 67.7%, 45.2%, 29.0%, 22.6%, 12.9%, and 3.2%, respectively. The positive rate of qacEDelta1-sul1 and intl1 were 80.6% and 58.1%, respectively. Six different PFGE clones were found, of which two dominated. The findings show that clinical isolates of MDRAB harbor various kinds of resistance genes.
使用聚合酶链反应（PCR）对31株多重耐药鲍曼不动杆菌（MDRAB）临床分离株的β-内酰胺酶（BLA）基因、氨基糖苷类修饰酶（AMEs）基因、消毒剂-磺胺耐药（qacEDelta1-sul1）基因、1类整合酶（intl1）基因以及与质粒介导喹诺酮耐药相关的qnr基因进行分析，并通过DNA测序进行验证。采用脉冲场凝胶电泳（PFGE）进行菌株分型。BLA基因中ADC、TEM、PER和DHA的阳性率分别为100%、61.3%、19.4%和3.2%；然而，SHV、OXA-23组、OXA-24组、GES、VIM、IMP和qnr基因均为阴性。AMEs基因中aac(3)-I、aac(6')-I、ant(3")-I、ant(2")-I、aac(3)-II和aac(6')-II的阳性率分别为67.7%、45.2%、29.0%、22.6%、12.9%和3.2%。qacEDelta1-sul1和intl1的阳性率分别为80.6%和58.1%。发现了6种不同的PFGE克隆，其中两种占主导地位。研究结果表明，MDRAB临床分离株携带多种耐药基因。
