Oz Carla, Zaken Neomi, Amiel Merav, Zamir Ashira
DNA Database Laboratory, Division of Identification and Forensic Science, Israel Police, National H.Q., Jerusalem, Israel.
J Forensic Sci. 2008 Jul;53(4):858-61. doi: 10.1111/j.1556-4029.2008.00761.x.
Autosomal short tandem repeat (STR) analysis has become highly relevant in the identification of victims from mass disasters and terrorist attacks. In such events, gender misidentification can be of grave consequences, yet the list reporting amelogenin amplification failure using STR multiplex kits continues to grow. Presented here are three such examples. In the first case, we present two male suspects who demonstrated amelogenin Y-deficient results using two commercial kit procedures. The presence of their Y chromosomes was proven by obtaining a Y-haplotype. The second case demonstrated a profile from a third male suspect where only the Y homolog of the XY pair was amplified. In events such as mass disasters or terrorist attacks, timely and reliable high throughput DNA typing results are essential. As the number of reported cases of amplification failure at the amelogenin gene continues to grow, we suggest that the incorporation of a better gender identification tool in commercial kits is crucial.
常染色体短串联重复序列（STR）分析在大规模灾难和恐怖袭击受害者的身份鉴定中变得至关重要。在这类事件中，性别误判可能会造成严重后果，然而，使用STR复合扩增试剂盒报告牙釉蛋白扩增失败的案例仍在不断增加。这里展示了三个这样的例子。在第一个案例中，我们呈现了两名男性嫌疑人，他们使用两种商业试剂盒程序均显示出牙釉蛋白Y基因缺失的结果。通过获得Y单倍型证实了他们Y染色体的存在。第二个案例展示了第三名男性嫌疑人的图谱，其中仅扩增出XY对中的Y同源物。在大规模灾难或恐怖袭击等事件中，及时且可靠的高通量DNA分型结果至关重要。随着牙釉蛋白基因扩增失败报告案例数量的持续增加，我们建议在商业试剂盒中纳入更好的性别鉴定工具至关重要。
