Kanzaki T, Kitajima S, Suzumori K
Department of Dermatology, Nagoya City University Medical School, Japan.
Cancer Res. 1991 Apr 15;51(8):2133-7.
A human malignant fibrous histiocytoma cell line was established in vitro. Cells showed a wide variety of morphologies, although the karyotype study showed that the tumor was monoclonal in origin because of the presence of unique marker chromosomes in 100% of the cells examined (50 of 50). Cells were cloned according to their characteristic morphologies and biological behavior in culture. The cloned cells were sparse spindle, packed spindle, epithelioid, and lymphoid. In colonies, sparse spindle cells grew separately from each other without cell to cell contact but produced a cartwheel pattern at confluency. Packed spindle cells grew in a tightly packed fashion and produced a storiform pattern at confluency. Epithelioid cells were spindle shaped as individuals but became epithelioid when in contact with each other and produced many multinucleated giant cells at confluency. Lymphoid cells were spindle shaped as individuals but became spherical at confluency. When tumors were grown in nude mice after transplantation of these cloned cells, the histology was shown to be unrelated to morphology in culture and was epithelioid (histiocytic), as was the original tumor. These results show that (a) a single cell derived from malignant fibrous histiocytoma cells exhibits a wide range of phenotypical expression in vitro, (b) cells have their own morphological and biological characteristics in vitro, which (c) however, are easily influenced by environmental factors and (d) which are unstable and even interchangeable. These characteristics may contribute to the endless variety of cellular forms and growth patterns of malignant fibrous histiocytomas in humans.
一株人恶性纤维组织细胞瘤细胞系在体外建立。细胞呈现出多种形态，尽管核型研究表明该肿瘤起源于单克隆，因为在检查的所有细胞（50个细胞中的50个）中都存在独特的标记染色体。根据细胞在培养中的特征形态和生物学行为进行克隆。克隆的细胞有稀疏梭形、密集梭形、上皮样和淋巴样。在集落中，稀疏梭形细胞彼此分开生长，细胞间无接触，但在汇合时形成车轮状图案。密集梭形细胞紧密排列生长，汇合时形成席纹状图案。上皮样细胞单个时呈梭形，但相互接触时变为上皮样，汇合时产生许多多核巨细胞。淋巴样细胞单个时呈梭形，但汇合时变为球形。将这些克隆细胞移植到裸鼠体内生长肿瘤后，组织学显示与培养中的形态无关，为上皮样（组织细胞样），与原发肿瘤相同。这些结果表明：（a）源自恶性纤维组织细胞瘤细胞的单个细胞在体外表现出广泛的表型表达；（b）细胞在体外有其自身的形态和生物学特征；（c）然而，这些特征很容易受到环境因素的影响；（d）并且这些特征不稳定甚至可相互转换。这些特性可能导致人类恶性纤维组织细胞瘤细胞形态和生长模式的无穷变化。
