Yonemoto T, Takenouchi T, Tokita H, Tatezaki S, Mukaida N, Mikata A, Moriya H
Department of Orthopaedic Surgery, Chiba University, Japan.
Clin Orthop Relat Res. 1995 Nov(320):159-67.
Malignant fibrous histiocytoma is the most frequent soft tissue sarcoma. However, the pathogenesis still remains unclear, because there are very few human malignant fibrous histiocytoma cell lines available for precise cellular study. In this study, a human malignant fibrous histiocytoma cell line (MMF-1) was established from the pulmonary metastatic lesion of a 55-year-old man with malignant fibrous histiocytoma. Human cell line MMF-1 and its heterotransplanted tumor had almost the same characteristics as the original tumor morphologically and immunohistochemically. This cell line is expected to be a useful for studying the pathogenesis of malignant fibrous histiocytoma. The cloned cell lines (MMF-2 and MMF-3) also consisted of spindle-shaped, polygonal, and multinucleated giant cells, meaning that the fibroblast-like cells, histiocyte-like cells, and multinucleated giant cells seen in malignant fibrous histiocytoma were derived from a single tumor cell. Human cell line MMF-1 produced inflammatory cytokines, such as tumor necrosis factor-alpha, macrophage colony-stimulating factor, interleukin-8, and monocyte chemotactic and activating factor, that might be involved in the morphogenesis of malignant fibrous histiocytoma. Furthermore, the results of the analysis of human cell line MMF-1 suggested that malignant fibrous histiocytoma originated from a poorly differentiated fibroblast.
恶性纤维组织细胞瘤是最常见的软组织肉瘤。然而，其发病机制仍不清楚，因为可供精确细胞研究的人类恶性纤维组织细胞瘤细胞系非常少。在本研究中，从一名55岁恶性纤维组织细胞瘤男性患者的肺转移病灶中建立了一株人类恶性纤维组织细胞瘤细胞系（MMF-1）。人类细胞系MMF-1及其异种移植瘤在形态学和免疫组织化学方面与原发肿瘤几乎具有相同的特征。该细胞系有望用于研究恶性纤维组织细胞瘤的发病机制。克隆细胞系（MMF-2和MMF-3）也由梭形、多边形和多核巨细胞组成，这意味着在恶性纤维组织细胞瘤中所见的成纤维细胞样细胞、组织细胞样细胞和多核巨细胞均来源于单个肿瘤细胞。人类细胞系MMF-1产生炎性细胞因子，如肿瘤坏死因子-α、巨噬细胞集落刺激因子、白细胞介素-8和单核细胞趋化激活因子，这些因子可能参与恶性纤维组织细胞瘤的形态发生。此外，对人类细胞系MMF-1的分析结果表明，恶性纤维组织细胞瘤起源于低分化成纤维细胞。
