通过显微注射小反义RNA和DNA分子抑制SV40基因表达。
Inhibition of SV40 gene expression by microinjected small antisense RNA and DNA molecules.
作者信息
Graessmann M, Michaels G, Berg B, Graessmann A
机构信息
Institut für Molekularbiologie und Biochemie der Freien Universität Berlin, FRG.
出版信息
Nucleic Acids Res. 1991 Jan 11;19(1):53-8. doi: 10.1093/nar/19.1.53.
Abstract
We tested the impact of antisense RNA and DNA molecules on SV40 gene expression by microinjection into TC7 cells. Short antisense stretches, complementary to either hairpin or loop structures on the T antigen mRNA, inhibited T antigen synthesis. In contrast, full-length antisense RNA and DNA molecules did not effect T antigen synthesis.
摘要
我们通过显微注射到TC7细胞中，测试了反义RNA和DNA分子对SV40基因表达的影响。与T抗原mRNA上的发夹或环结构互补的短反义片段抑制了T抗原的合成。相比之下，全长反义RNA和DNA分子对T抗原的合成没有影响。
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