Hentzen D
Cancer Res. 1976 Sep;36(9 pt.1):3082-5.
Mammary gland transfer RNA's (tRNA'S) of CEH mice infected with mammary tumor virus were analyzed in the preneoplastic state and compared to tRNAs of virus-free C3Hf mice and another uninfected strain, C57BL/6, which is completely resistant to cancer. This quantitative study was based on the ability of each tRNA to fix its corresponding amino acid. The amount of each of the 17 tRNA's tested was identical for the three mammary glands. In addition, tRNA populations during lactation correlated with the amino acids incorporated into the lactoproteins synthesized, which indicates adapation of the tRNA's to protein biosynthesis. Qualitative chromatographic studies on reverse phase capillary columns Type 5 of 10 aminoacyl-tRNA's did not reveal any difference in the isoacceptor elution profiles. This shows that no new isoaccepting tRNA is associated with the mammary tumor virus at that stage, and that no viral modification of a host tRNA has occurred.
对感染乳腺肿瘤病毒的CEH小鼠的乳腺转移核糖核酸（tRNA）进行了肿瘤前状态分析，并与无病毒的C3Hf小鼠以及对癌症完全有抗性的另一个未感染品系C57BL/6的tRNA进行了比较。这项定量研究基于每种tRNA固定其相应氨基酸的能力。所测试的17种tRNA中，每种tRNA在三个乳腺中的含量相同。此外，哺乳期的tRNA群体与掺入所合成乳蛋白中的氨基酸相关，这表明tRNA适应了蛋白质生物合成。对10种氨酰tRNA在5型反相毛细管柱上进行的定性色谱研究未揭示同功受体洗脱图谱有任何差异。这表明在那个阶段没有新的同功受体tRNA与乳腺肿瘤病毒相关联，并且宿主tRNA没有发生病毒修饰。
