Bussolino F, Sordano C, Benfenati E, Bozzaro S
Department of Clinical and Biological Sciences, University of Turin, Torino, Italy.
Eur J Biochem. 1991 Mar 28;196(3):609-15. doi: 10.1111/j.1432-1033.1991.tb15856.x.
Evidence is provided that Dictyostelium discoideum cells produce 1-O-alkyl-2-delta-acetyl-O-sn-glycero-3-phosphocholine (platelet-activating factor, PAF). D. discoideum PAF has been characterized as being identical with mammalian platelet-activating factor, based on its stimulation of rabbit platelet aggregation, its physicochemical properties and mass spectrum. The basal activity of PAF increases after starvation and during aggregation and declines at the slug stage. PAF is not detected in the extracellular space. Cell treatment with cAMP pulses stimulates a transient accumulation of PAF, probably via activation of a cAMP-dependent acetyltransferase, suggesting a possible involvement of PAF in cAMP-regulated processes in Dictyostelium.
有证据表明，盘基网柄菌细胞可产生1-O-烷基-2-δ-乙酰基-O- sn -甘油-3-磷酸胆碱（血小板活化因子，PAF）。基于其对兔血小板聚集的刺激作用、理化性质和质谱分析，盘基网柄菌PAF已被鉴定为与哺乳动物血小板活化因子相同。PAF的基础活性在饥饿后和聚集过程中增加，而在蛞蝓阶段下降。在细胞外空间未检测到PAF。用cAMP脉冲处理细胞会刺激PAF的短暂积累，这可能是通过激活cAMP依赖性乙酰转移酶实现的，这表明PAF可能参与了盘基网柄菌中cAMP调节的过程。
