Chen Bor-Yann, Chen Wen-Ming, Kuo Hsiang-Yin, Hsueh Chung-Chuan
Department of Chemical and Materials Engineering National I-Lan University, Lan 260, Taiwan.
J Hazard Mater. 2009 Jan 15;161(1):526-33. doi: 10.1016/j.jhazmat.2008.03.138. Epub 2008 Apr 12.
This study provides a first attempt from a geological and ecological perspective to look forward isolations of indigenous strains with the decolorization capability from the most biodiverse region in Taiwan for dye-laden wastewater treatment. Serial selections were conducted by a specific use of the fungicide nystatin and model azo dye C.I. reactive red 141 (RR141) during isolation. Several bacterial strains with the excellent capability of azo dye decolorization were predominantly isolated from river water and mud samples of Lanyang River Basin. Phase-curve profiles indicated that azo dye decolorization was found to be non-growth associated for both mixed cultures and isolated pure strains. The color removal efficiency of the mixed culture was nearly 10-fold to that of Pseudomonas luteola at ca. 600mgL(-1) RR141, indicating a promising feasibility of isolated cultures to be applicable for practical treatments. The decolorization performance of unacclimated and acclimated pure cultures was at most 20% and 70-80% to that of the mixed cultures, respectively. It might suggest that combined interactions among decolorizers were crucial for the optimal color removal. According to the results of physiological and 16S rRNA gene sequence examinations, the isolated strains should belong to Aeromonas species (very likely A. hydrophila).
本研究首次从地质和生态角度出发，旨在从台湾生物多样性最高的地区筛选出具有脱色能力的本土菌株，用于处理含染料废水。在分离过程中，通过特定使用杀真菌剂制霉菌素和偶氮染料模型C.I.活性红141（RR141）进行连续筛选。从兰阳溪流域的河水和泥浆样本中主要分离出了几种具有优异偶氮染料脱色能力的细菌菌株。相曲线表明，对于混合培养物和分离出的纯菌株，偶氮染料脱色与生长无关。在约600mgL(-1) RR141浓度下，混合培养物的脱色效率几乎是淡黄假单胞菌的10倍，这表明分离出的培养物具有应用于实际处理的可行性。未驯化和驯化的纯培养物的脱色性能分别最多为混合培养物的20%和70 - 80%。这可能表明脱色剂之间的联合相互作用对于最佳脱色至关重要。根据生理和16S rRNA基因序列检测结果，分离出的菌株应属于气单胞菌属（很可能是嗜水气单胞菌）。
