Khalid Azeem, Arshad Muhammad, Crowley David E
Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, 38040, Pakistan.
Appl Microbiol Biotechnol. 2008 Feb;78(2):361-9. doi: 10.1007/s00253-007-1302-4. Epub 2007 Dec 15.
Wastewater effluents from the textile and other dye-stuff industries contain significant amounts of synthetic dyes that require treatment to prevent groundwater contamination. In research aimed at biotechnology for treatment of azo dyes, this study examined 288 strains of azo-dye degrading bacteria to identify efficient strains and determine incubation times required for decolorization. Initial enrichment cultures were carried out using a mixture of four structurally different dyes (Acid Red 88, Reactive Black 5, Direct Red 81, and Disperse Orange 3) as the sole source of C and N to isolate the bacteria from soil, activated sludge, and natural asphalt. Six strains were selected for further study based on their prolific growth and ability to rapidly decolorize the dyes individually or in mixtures. Treatment times required by the most efficient strain, AS96 (Shewanella putrefaciens) were as short as 4 h for complete decolorization of 100 mg l(-1) of AR-88 and DR-81 dyes under static conditions, and 6 and 8 h, respectively, for complete decolorization of RB-5 and DO-3. To our knowledge, these bacterial strains are the most efficient azo-dye degrading bacteria that have been described and may have practical application for biological treatment of dye-polluted wastewater streams.
纺织及其他染料工业产生的废水含有大量合成染料，需要进行处理以防止地下水污染。在旨在利用生物技术处理偶氮染料的研究中，本研究检测了288株偶氮染料降解菌，以鉴定高效菌株并确定脱色所需的培养时间。最初的富集培养使用四种结构不同的染料（酸性红88、活性黑5、直接红81和分散橙3）的混合物作为唯一的碳源和氮源，从土壤、活性污泥和天然沥青中分离细菌。根据它们的大量生长以及单独或混合快速使染料脱色的能力，选择了6株菌株进行进一步研究。最有效的菌株AS96（腐败希瓦氏菌）在静态条件下，使100 mg l(-1)的AR - 88和DR - 81染料完全脱色所需的处理时间短至4小时，使RB - 5和DO - 3完全脱色分别需要6小时和8小时。据我们所知，这些菌株是已报道的最有效的偶氮染料降解菌，可能在生物处理染料污染废水流方面具有实际应用价值。
