Khrestchatisky M, MacLennan A J, Tillakaratne N J, Chiang M Y, Tobin A J
Department of Biology, University of California, Los Angeles 90024-1606.
J Neurochem. 1991 May;56(5):1717-22. doi: 10.1111/j.1471-4159.1991.tb02072.x.
A cDNA from a rat hippocampal cDNA library encodes an isoform of the alpha polypeptide of the gamma-aminobutyric acid (GABA)/benzodiazepine (BZ) receptor. Its deduced amino acid sequence is 96% identical to that of the alpha 2 polypeptide of the bovine GABAA receptor. The polypeptide has features shared by all previously reported GABAA receptor alpha polypeptides and shares 71-76% identity with previously described rat alpha polypeptides. Most of the differences lie in the presumed extracellular and intracellular domains. On Northern blots, the alpha 2 cDNA detects two mRNAs, which are found in cortex, hippocampus, and striatum, brain regions enriched in pharmacologically defined "BZ type II" receptors. Other workers have previously shown that the alpha polypeptides of the GABAA receptor largely determine the BZ binding properties of reconstituted receptors. The distribution of alpha 2 mRNAs in rat brain suggests that the alpha 2 subunit may indeed be involved in the BZ type II receptors.
从大鼠海马cDNA文库中获得的一个cDNA编码γ-氨基丁酸（GABA）/苯二氮䓬（BZ）受体α多肽的一种亚型。其推导的氨基酸序列与牛GABAA受体α2多肽的序列有96%的同一性。该多肽具有所有先前报道的GABAA受体α多肽共有的特征，并且与先前描述的大鼠α多肽有71-76%的同一性。大多数差异存在于推测的细胞外和细胞内结构域。在Northern印迹上，α2 cDNA检测到两种mRNA，它们存在于皮质、海马和纹状体中，这些脑区富含药理学定义的“BZ II型”受体。其他研究人员先前已经表明，GABAA受体的α多肽在很大程度上决定了重组受体的BZ结合特性。α2 mRNA在大鼠脑中的分布表明α2亚基可能确实参与了BZ II型受体。
