Hammer J G, Howland D R
Medical College of Pennsylvania, Philadelphia 19129.
Lab Anim Sci. 1991 Jan;41(1):42-5.
There has been much research studying the effect of homotypic embryonic neural tissue transplantation in the central nervous system of rats and cats, with promising results. The benefits of transplantation are dependent upon the appropriate aged donor. Therefore, it is critical that the exact age of the embryonic tissue be known. For this reason we have developed a method of determining, indirectly, the pregnancy status and embryonic age in the domestic cat (Felis catus) using serum progesterone levels. Serum progesterone levels were monitored in 16 domestic cats during 26 breeding trials. Blood samples were taken prior to and 6 days after natural breeding to determine if ovulation had occurred, indirectly indicating pregnancy status. By knowing the exact date bred and if it resulted in pregnancy, an accurate embryonic age was calculated. A day 6 post-breeding serum progesterone concentration of less than 5.0 ng/ml was considered a negative indication of pregnancy, greater than 5.0 ng/ml a positive indication of pregnancy. Pregnancy was confirmed by abdominal palpation 21 to 26 days after breeding. Serum progesterone levels taken on the sixth day after observed breeding provide an accurate, indirect evaluation of pregnancy in the timed pregnant domestic cat (81%, p = 0.003).
已经有许多研究探讨了同种型胚胎神经组织移植在大鼠和猫中枢神经系统中的作用，结果很有前景。移植的益处取决于合适年龄的供体。因此，了解胚胎组织的确切年龄至关重要。出于这个原因，我们开发了一种利用血清孕酮水平间接确定家猫（Felis catus）妊娠状态和胚胎年龄的方法。在26次繁殖试验中，对16只家猫的血清孕酮水平进行了监测。在自然交配前和交配后6天采集血样，以确定是否发生排卵，从而间接表明妊娠状态。通过知道确切的交配日期以及是否受孕，计算出准确的胚胎年龄。交配后第6天血清孕酮浓度低于5.0 ng/ml被认为是妊娠阴性指征，高于5.0 ng/ml则为妊娠阳性指征。在交配后21至26天通过腹部触诊确认妊娠。观察到交配后第6天采集的血清孕酮水平为定时妊娠的家猫的妊娠情况提供了准确的间接评估（81%，p = 0.003）。
