Murphy D G, Dimock K, Kang C Y
Department of Microbiology and Immunology, University of Ottawa, Faculty of Medicine, Ontario, Canada.
Virology. 1991 Apr;181(2):760-3. doi: 10.1016/0042-6822(91)90913-v.
The nucleotide sequences at the 3'-termini of human parainfluenza virus 3 (HPIV3) genomic RNAs recovered from two lines of persistently infected LLC-MK2 cells were determined following PCR amplification. After 29 months of persistence the 3'-end of the HPIV3 genome was found to be highly mutated. Interestingly, the only types of nucleotide changes observed were U to C and A to G transitions. Both U to C and A to G transitions were present on individual RNA molecules. The data indicate that biased hypermutational activity leading to U To C and A to G mutations operates in cultured cells during persistent HPIV3 infections.
在进行PCR扩增后，测定了从两株持续感染的LLC-MK2细胞系中回收的人副流感病毒3型（HPIV3）基因组RNA 3'末端的核苷酸序列。持续感染29个月后，发现HPIV3基因组的3'末端发生了高度突变。有趣的是，观察到的唯一核苷酸变化类型是U到C和A到G的转换。U到C和A到G的转换都存在于单个RNA分子上。数据表明，在持续的HPIV3感染期间，导致U到C和A到G突变的偏向性超突变活性在培养细胞中起作用。
