Scieller P, Omilli F, Borde J, May E
Laboratoire d'Oncologie Moléculaire, Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.
Virology. 1991 Apr;181(2):783-6. doi: 10.1016/0042-6822(91)90918-2.
By analyzing the late promoter activity of a series of nonreplicative recombinants mutated within the different enhancer motifs of SV40 we identified both positive and negative regulatory elements. In the absence of T-antigen, the motifs Sph and/or octamer, and to a lesser extent the motifs GTI and P, account for the constitutive expression of the late promoter. The motif GTII overlaps elements that negatively regulate the expression of the late promoter. These results indicate that the late promoter is down-regulated not only at the level of the GC motifs but also at the enhancer level. Moreover, we showed that T-antigen interacts with both positive and negative regulatory elements.
通过分析一系列在SV40不同增强子基序内发生突变的非复制性重组体的晚期启动子活性，我们鉴定出了正调控元件和负调控元件。在没有T抗原的情况下，Sph和/或八聚体基序，以及程度稍小的GTI和P基序，负责晚期启动子的组成型表达。GTII基序与负调控晚期启动子表达的元件重叠。这些结果表明，晚期启动子不仅在GC基序水平受到下调，在增强子水平也受到下调。此外，我们还表明T抗原与正调控元件和负调控元件都相互作用。
