Pozharisskiĭ K M, Nikitin A Iu
Vopr Onkol. 1991;37(1):51-9.
Light and electron microscopy, histochemistry, histoautoradiography and vital staining were used to assess changes occurring in subcutaneous connective tissue surrounding a DMBA-containing pill in rats, samples being obtained at 2-week intervals until malignant fibrous histiocytoma became apparent. Initial tumor lesions developing against the background of alterative-inflammatory and reparative changes included apparently atypical pleomorphic cells showing cytologic signs of malignant transformation which remained virtually unchanged in developed sarcoma. The study, therefore, failed to identify the stage of "presarcoma". A complex of morphologic and functional characteristics of the atypical cells revealed their fibroblastic nature. Progression of tumor was associated with appearance of cells of histiocytic pattern (as evidenced by high activity of acid phosphatase and non-specific esterases, thin villus-like outgrowths of cellular membrane and well-developed Golgi complex) which pointed to pronounced structural and functional variability of poorly-differentiated neoplastic fibroblasts. Tumor revealed xanthomatous and multinuclear giant cells, too. Malignant fibrous histiocytoma is, therefore, poorly-differentiated polymorphocellular sarcoma of fibroblastic origin.
采用光镜和电镜检查、组织化学、组织放射自显影术及活体染色法，对大鼠含二甲基苯并蒽药丸周围皮下结缔组织发生的变化进行评估，每隔2周取材一次，直至明显出现恶性纤维组织细胞瘤。在交替性炎症和修复性变化的背景下出现的初始肿瘤病变，包括明显非典型的多形性细胞，这些细胞显示出恶性转化的细胞学特征，在已形成的肉瘤中基本保持不变。因此，该研究未能确定“肉瘤前期”阶段。非典型细胞的形态和功能特征综合显示出它们的成纤维细胞性质。肿瘤的进展与组织细胞样模式细胞的出现有关（酸性磷酸酶和非特异性酯酶活性高、细胞膜有细绒毛样突起以及高尔基体发达可证明），这表明低分化肿瘤性成纤维细胞具有明显的结构和功能变异性。肿瘤中还可见黄色瘤细胞和多核巨细胞。因此，恶性纤维组织细胞瘤是一种起源于成纤维细胞的低分化多形性细胞肉瘤。
