Elghetany M T, Kurec A S, Schuehler K, Forbes B A, Duggan D B, Davey F R
Department of Pathology, State University of New York Health Science Center, Syracuse 13210.
Am J Clin Pathol. 1991 Apr;95(4):517-25. doi: 10.1093/ajcp/95.4.517.
Several monoclonal antibodies (MoAbs) are now available for immunophenotyping non-Hodgkin's lymphomas (NHLs) in paraffin-embedded tissue sections. To determine the reliability of these reagents in predicting the genotype, 44 cases of NHL were studied with the alkaline phosphatase-anti-alkaline phosphatase technique with the use of the following MoAbs: leukocyte common antigen (CD45), Mac 387, L26, 4KB5, MB1, MB2, LN2, UCHL1, MT1, and MT2. The lineage of the neoplastic cells was determined in all cases by gene rearrangement studies for immunoglobulin heavy chain and for the T-cell receptor beta-chain. Genotypic results showed B-cell lineage in 33 cases (75%), T-cell lineage in 6 cases (14%), and mixed or undetermined lineage in 5 cases (11%). A concordance of lineage assignment by paraffin section immunophenotyping with gene rearrangement studies was observed in 37 of 39 (95%) lymphomas with an unequivocally defined genotype. MoAb L26 was the most sensitive in detecting B-cell genotype; MoAbs MT1 and UCHL1 were the most sensitive and specific, respectively, in detecting T-cell genotype. The authors conclude that lineage assignment of NHLs in paraffin sections is reflective of the corresponding genotype when an appropriate panel of MoAbs is used.
目前有几种单克隆抗体（MoAbs）可用于对石蜡包埋组织切片中的非霍奇金淋巴瘤（NHLs）进行免疫表型分析。为了确定这些试剂在预测基因型方面的可靠性，使用以下MoAbs通过碱性磷酸酶-抗碱性磷酸酶技术对44例NHL进行了研究：白细胞共同抗原（CD45）、Mac 387、L26、4KB5、MB1、MB2、LN2、UCHL1、MT1和MT2。通过对免疫球蛋白重链和T细胞受体β链进行基因重排研究，确定了所有病例中肿瘤细胞的谱系。基因型结果显示，33例（75%）为B细胞谱系，6例（14%）为T细胞谱系，5例（11%）为混合或未确定谱系。在39例基因型明确的淋巴瘤中，有37例（95%）观察到石蜡切片免疫表型分析与基因重排研究在谱系判定上具有一致性。MoAb L26在检测B细胞基因型方面最为敏感；MoAbs MT1和UCHL1分别在检测T细胞基因型方面最为敏感和特异。作者得出结论，当使用合适的MoAbs组合时，石蜡切片中NHLs的谱系判定反映了相应的基因型。
