Katzin W E, Linden M D, Fishleder A J, Tubbs R R
Department of Laboratory Hematology, Cleveland Clinic Foundation, Ohio 44195-5138.
Am J Pathol. 1989 Oct;135(4):615-21.
Diffuse, mixed small and large cell lymphomas (DML) are a heterogeneous group of non-Hodgkin's lymphomas. There are only a few published immunophenotypic and/or genotypic studies of DML, and they include a small number of cases. It is unclear whether these neoplasms are monoclonal, oligoclonal, or perhaps of dual lineage. Using monoclonal antibodies (UCHL1, MB2, MT1, LN1, LN2, and L26) that are effective in paraffin-embedded, B5-fixed tissue, 13 cases of DML were studied. This method allowed for improved correlation between cell morphology and immunophenotype compared with frozen section immunohistology. In addition, Southern blotting/DNA hybridization was used to identify directly the lineage of the neoplastic cell population. In each case a population of large B lymphocytes was demonstrated by immunohistology. In six of the cases, a single class of immunoglobulin light chains was detected by frozen section immunohistology, cytospin immunocytology, or both supporting the hypothesis that the B lymphocytes are monoclonal. In almost all the cases (12 of 13), the small cell population consisted predominantly of T lymphocytes. Immunoglobulin gene rearrangements were detected in seven cases, but no T cell receptor gene rearrangements were detected. It was concluded that DML are monoclonal lymphomas of B cell lineage with a non-neoplastic T cell component.
弥漫性混合小细胞和大细胞淋巴瘤（DML）是一组异质性非霍奇金淋巴瘤。关于DML的免疫表型和/或基因型研究仅有少数发表，且病例数量较少。目前尚不清楚这些肿瘤是单克隆性、寡克隆性，还是可能为双谱系。使用对石蜡包埋、B5固定组织有效的单克隆抗体（UCHL1、MB2、MT1、LN1、LN2和L26），对13例DML进行了研究。与冰冻切片免疫组织化学相比，该方法可改善细胞形态与免疫表型之间的相关性。此外，采用Southern印迹/DNA杂交直接鉴定肿瘤细胞群体的谱系。通过免疫组织化学在每例中均证实存在一群大B淋巴细胞。在6例病例中，通过冰冻切片免疫组织化学、细胞离心涂片免疫细胞化学或两者均检测到单一类别的免疫球蛋白轻链，支持B淋巴细胞为单克隆性的假说。在几乎所有病例（13例中的12例）中，小细胞群体主要由T淋巴细胞组成。在7例中检测到免疫球蛋白基因重排，但未检测到T细胞受体基因重排。得出的结论是，DML是具有非肿瘤性T细胞成分的B细胞谱系单克隆淋巴瘤。
