Sticher Otto
Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology, Zurich 8093, Switzerland.
Nat Prod Rep. 2008 Jun;25(3):517-54. doi: 10.1039/b700306b. Epub 2008 Mar 11.
Since the 1990s, interest in natural product research has increased considerably. Following several outstanding developments in the areas of separation methods, spectroscopic techniques, and sensitive bioassays, natural product research has gained new attention for providing novel chemical entities. This updated review deals with sample preparation and purification, recent extraction techniques used for natural product separation, liquid-solid and liquid-liquid isolation techniques, as well as multi-step chromatographic operations. It covers examples of papers published since the NPR review 'Modern separation methods' by Marston and Hostettmann,1 with major emphasis on methods developed and the research undertaken since 2000.
自20世纪90年代以来，对天然产物研究的兴趣大幅增加。随着分离方法、光谱技术和灵敏生物测定领域的几项杰出进展，天然产物研究因提供新型化学实体而重新受到关注。这篇更新的综述涉及样品制备与纯化、用于天然产物分离的最新提取技术、液固和液液分离技术以及多步色谱操作。它涵盖了自马斯顿和霍斯特特曼发表天然产物研究综述《现代分离方法》以来发表的论文实例，主要侧重于2000年以来开发的方法和开展的研究。
