Torres Cristian, Antileo Elmer, Epuñán Maráa José, Pino Ana María, Valladares Luis Emilio, Sierralta Walter Daniel
Laboratorio de Hormonas y Receptores, INTA-Universidad de Chile, Santiago, Chile.
Oncol Rep. 2008 Jun;19(6):1597-603.
A cyclic peptide derived from the active domain of alpha-fetoprotein (AFP) significantly inhibited the proliferation of MCF7 cells stimulated with the epidermal growth factor (EGF) or estradiol (E2). The action of these three agents on cell growth was independent of the presence of calf serum in the culture medium. Our results demonstrated that the cyclic peptide interfered markedly with the regulation of MAPK by activated c-erbB2. The cyclic peptide showed no effect on the E2-stimulated release of matrix metalloproteinases 2 and 9 nor on the shedding of heparin-binding EGF into the culture medium. We propose that the AFP-derived cyclic peptide represents a valuable novel antiproliferative agent for treating breast cancer.
一种源自甲胎蛋白（AFP）活性结构域的环肽，可显著抑制由表皮生长因子（EGF）或雌二醇（E2）刺激的MCF7细胞的增殖。这三种物质对细胞生长的作用与培养基中是否存在小牛血清无关。我们的结果表明，该环肽通过激活的c-erbB2显著干扰了丝裂原活化蛋白激酶（MAPK）的调节。该环肽对E2刺激的基质金属蛋白酶2和9的释放以及肝素结合表皮生长因子向培养基中的脱落均无影响。我们认为，源自AFP的环肽是一种用于治疗乳腺癌的有价值的新型抗增殖剂。
