从金属污染废水中分离出的芽孢杆菌属ev3的铬耐受性及还原电位
Chromium tolerance and reduction potential of a Bacillus sp.ev3 isolated from metal contaminated wastewater.
作者信息
Rehman A, Zahoor A, Muneer B, Hasnain S
机构信息
Department of Microbiology and Molecular Genetics, University of the Punjab, New Campus, Lahore 54590, Pakistan.
出版信息
Bull Environ Contam Toxicol. 2008 Jul;81(1):25-9. doi: 10.1007/s00128-008-9442-5. Epub 2008 May 23.
This study was aimed at assessing the ability of Bacillus sp.ev3 to reduce hexavalent chromium into its trivalent form. Bacillus sp.ev3 could tolerate Cr(6+) (4800 microg/mL), Pb(2+) (800 microg/mL), Cu(2+) (200 microg/mL), Cd(2+) (50 microg/mL), Zn(2+) (400 microg/mL), Ni(2+) (4000 microg/mL) and Hg(2+) (50 microg/mL). Bacillus sp.ev3 showed optimum growth at 37 degrees C and pH at 7. Bacillus sp.ev3 could reduce 91% of chromium from the medium after 96 h and was also capable to reduce 84% chromium from the industrial effluents after 144 h. Cell free extracts of Bacillus sp.ev3 grown in the presence of Cr showed reduction of 70%, 45.6% and 27.4% at concentrations of 10 microg Cr(6+)/mL, 50 microg Cr(6+)/mL and 100 microg Cr(6+)/mL, respectively.
本研究旨在评估芽孢杆菌ev3将六价铬还原为三价铬的能力。芽孢杆菌ev3能够耐受Cr(6+)（4800微克/毫升）、Pb(2+)（800微克/毫升）、Cu(2+)（200微克/毫升）、Cd(2+)（50微克/毫升）、Zn(2+)（400微克/毫升）、Ni(2+)（4000微克/毫升）和Hg(2+)（50微克/毫升）。芽孢杆菌ev3在37摄氏度和pH值为7时生长最佳。芽孢杆菌ev3在96小时后可将培养基中91%的铬还原，在144小时后也能够将工业废水中84%的铬还原。在含有铬的条件下生长的芽孢杆菌ev3的无细胞提取物，在铬(6+)浓度为10微克/毫升、50微克/毫升和100微克/毫升时，分别显示出70%、45.6%和27.4%的还原率。
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