Liu Jianghong, Xue Jian, Wei Xiaohang, Su Huimin, Xu Ruidan
Provincial Key Laboratory of Oil & Gas Chemical Technology, College of Chemistry & Chemical Engineering, Northeast Petroleum University, Daqing, 163318 Heilongjiang China.
School of Life Sciences, Peking University, Beijing, 100871 China.
Indian J Microbiol. 2020 Dec;60(4):430-435. doi: 10.1007/s12088-020-00886-3. Epub 2020 May 23.
The removal rate of Cr has been explored by the optimized removal conditions. Five Cr-resistant strains were isolated from chromium-contained soil. The most efficient strain S1 was identified as strain SZMC 6179J through 16S rDNA. Response surface methodology (RSM) was used to investigate the effects of four independent variables, including initial pH, initial Cr concentration (mg/L), time (h) and inoculation percentage (%). RSM revealed that when pH was 5.02, time was 24.0 h, inoculation percentage was 4.64% (v/v) and initial concentration of Cr was 55.0 mg/L, the optimal condition was obtained. Under the optimum conditions, the actual response values for strain SZMC 6179J was 93.50%. The pH was the most significant factor towards removal rate of Cr. The result showed that the removal mechanism of Cr by strain SZMC 6179J was reduction under normal conditions. The removal mechanism of Cr by strain SZMC 6179J was adsorption under adverse conditions.
通过优化的去除条件对铬的去除率进行了探究。从含铬土壤中分离出了5株耐铬菌株。通过16S rDNA将最有效的菌株S1鉴定为菌株SZMC 6179J。采用响应面法（RSM）研究了初始pH值、初始铬浓度（mg/L）、时间（h）和接种百分比（%）这四个自变量的影响。RSM结果表明，当pH值为5.02、时间为24.0 h、接种百分比为4.64%（v/v）且初始铬浓度为55.0 mg/L时，可获得最佳条件。在最佳条件下，菌株SZMC 6179J的实际响应值为93.50%。pH值是影响铬去除率的最显著因素。结果表明，在正常条件下，菌株SZMC 6179J对铬的去除机制为还原作用。在不利条件下，菌株SZMC 6179J对铬的去除机制为吸附作用。
