Lundry Denise R, Ridley William P, Meyer Jiaying J, Riordan Susan G, Nemeth Margaret A, Trujillo William A, Breeze Matthew L, Sorbet Roy
Monsanto Company, 800 North Lindbergh Boulevard, St. Louis, Missouri 63167, USA.
J Agric Food Chem. 2008 Jun 25;56(12):4611-22. doi: 10.1021/jf073087h. Epub 2008 May 23.
Developments in biotechnology and molecular-assisted breeding have led to the development of a second-generation glyphosate-tolerant soybean product, MON 89788. The MON 89788 event was produced by direct transformation of a cp4 epsps (5-enolpyruvylshikimate-3-phosphate synthase) gene cassette derived from Agrobacterium sp. strain CP4 into an elite soybean germplasm known for its superior agronomic characteristics and high yielding property. The purpose of this work was to assess whether the nutrient and antinutrient levels in seed and forage tissues of MON 89788 are comparable to those in the conventional soybean variety, A3244, which has background genetics similar to MON 89788 but does not contain the cp4 epsps gene cassette. Additional conventional soybean varieties currently in the marketplace were also included in the analysis to establish a range of natural variability for each analyte, where the range of variability is defined by a 99% tolerance interval for that particular analyte. Compositional analyses were conducted on forage, seed and four processed fractions from soybeans grown in ten sites across both the United States and Argentina during the 2004-2005 growing seasons. Forage samples were analyzed for levels of proximates (ash, fat, moisture, and protein) and fiber. Seed samples were analyzed for proximates, fiber, antinutrients, and vitamin E. Defatted, toasted (DT) meal was analyzed for proximates, fiber, amino acids, and antinutrients. Refined, bleached, and deodorized (RBD) oil was analyzed for fatty acids and vitamin E. Protein isolate was analyzed for amino acids and moisture. Crude Lecithin was analyzed for phosphatides. Results of the comparisons indicate that MON 89788 is compositionally and nutritionally equivalent to conventional soybean varieties currently in commerce.
生物技术和分子辅助育种的发展催生了第二代耐草甘膦大豆产品MON 89788。MON 89788事件是通过将源自土壤杆菌属CP4菌株的cp4 epsps(5-烯醇丙酮酰莽草酸-3-磷酸合酶)基因盒直接转化到一种优良大豆种质中产生的,该种质以其优良的农艺性状和高产特性而闻名。这项工作的目的是评估MON 89788种子和饲料组织中的营养成分和抗营养成分水平是否与传统大豆品种A3244相当,A3244的背景遗传与MON 89788相似,但不包含cp4 epsps基因盒。分析中还纳入了目前市场上的其他传统大豆品种,以确定每种分析物的自然变异范围,变异范围由该特定分析物的99%耐受区间定义。在2004 - 2005生长季,对在美国和阿根廷十个地点种植的大豆的饲料、种子和四个加工组分进行了成分分析。对饲料样本分析了常规成分(灰分、脂肪、水分和蛋白质)和纤维。对种子样本分析了常规成分、纤维、抗营养成分和维生素E。对脱脂烤粕分析了常规成分、纤维、氨基酸和抗营养成分。对精炼、漂白和脱臭(RBD)油分析了脂肪酸和维生素E。对蛋白质分离物分析了氨基酸和水分。对粗卵磷脂分析了磷脂。比较结果表明,MON 89788在成分和营养上与目前商业上的传统大豆品种相当。