杆状病毒基因组的直接双链DNA测序

Direct double-stranded DNA sequencing with baculovirus genomes.

作者信息

Wang H H, Fraser M J

机构信息

Department of Biological Sciences, University of Notre Dame, Indiana 46556.

出版信息

J Virol Methods. 1991 Jan;31(1):113-8. doi: 10.1016/0166-0934(91)90149-t.

DOI:10.1016/0166-0934(91)90149-t

PMID:1849913

Abstract

Double-stranded DNA sequencing with the modified T7 DNA polymerase (Sequenase) was performed directly with nuclear polyhedrosis virus DNA genomes. The conditions were optimized to allow for a rapid and unambiguous sequence analysis of nuclear polyhedrosis virus genomes.

摘要

使用改良的T7 DNA聚合酶（测序酶）对核型多角体病毒DNA基因组直接进行双链DNA测序。对条件进行了优化，以便能够对核型多角体病毒基因组进行快速且明确的序列分析。