Simão Andréa Name Colado, Dichi Jane Bandeira, Barbosa Décio Sabbatini, Cecchini Rubens, Dichi Isaias
Department of Pathology, Clinical Analysis and Toxicology, University of Londrina, Londrina, Paraná, Brazil.
Nutrition. 2008 Jul-Aug;24(7-8):675-81. doi: 10.1016/j.nut.2008.03.021. Epub 2008 May 21.
Metabolic syndrome (MS) is a cluster of risk factors for cardiovascular disease related mainly to insulin resistance, but also to oxidative stress. Uric acid and gamma-glutamyltransferase (GGT) levels are also associated with MS and oxidative stress. This study was undertaken to assess the role of GGT and uric acid in adult patients with MS and its relation to oxidative stress and antioxidant defense.
A total of 88 adults (67 with MS and 21 controls) were selected among ambulatory patients and workers of the University Hospital of Londrina, Paraná, Brazil. Oxidative stress was assessed by determination of thiobarbituric acid-reactive substances and tert-butyl hydroperoxide-initiated chemiluminescence and antioxidant defenses by total radical-trapping antioxidant parameter.
The MS group presented higher significant results (P < 0.0001) than the control group in all parameters of MS and uric acid and GGT levels and significant lower values (P < 0.0001) in high-density lipoprotein cholesterol levels. Thiobarbituric acid-reactive substances and total radical-trapping antioxidant parameter did not show statistically significant differences between groups. However, lipid hydroperoxides, evaluated by tert-butyl hydroperoxide-initiated chemiluminescence, showed higher significant results in the MS group (P = 0.045) than in the control group. Total antioxidant capacity did not decrease and thiobarbituric acid-reactive substances did not increase, probably due to increased uric acid (r = 0.239, P = 0.04) in the MS group.
The present study confirmed that GGT is a strong predictor of MS and that lipid peroxide measured by tert-butyl hydroperoxide-initiated chemiluminescence and GGT activity are reliable markers of oxidative stress in this syndrome.
代谢综合征（MS）是一组心血管疾病风险因素，主要与胰岛素抵抗相关，但也与氧化应激有关。尿酸和γ-谷氨酰转移酶（GGT）水平也与MS及氧化应激相关。本研究旨在评估GGT和尿酸在成年MS患者中的作用及其与氧化应激和抗氧化防御的关系。
从巴西巴拉那州隆德里纳大学医院的门诊患者和工作人员中选取了88名成年人（67名MS患者和21名对照）。通过测定硫代巴比妥酸反应性物质和叔丁基过氧化氢引发的化学发光来评估氧化应激，并通过总自由基捕获抗氧化参数评估抗氧化防御。
MS组在所有MS参数、尿酸和GGT水平方面的结果均显著高于对照组（P < 0.0001），而高密度脂蛋白胆固醇水平显著低于对照组（P < 0.0001）。硫代巴比妥酸反应性物质和总自由基捕获抗氧化参数在两组之间未显示出统计学显著差异。然而，通过叔丁基过氧化氢引发的化学发光评估的脂质过氧化物在MS组中的结果显著高于对照组（P = 0.045）。MS组总抗氧化能力未降低，硫代巴比妥酸反应性物质也未增加，可能是由于尿酸增加（r = 0.239，P = 0.04）。
本研究证实GGT是MS的一个强预测指标，并且通过叔丁基过氧化氢引发的化学发光测定的脂质过氧化物和GGT活性是该综合征氧化应激的可靠标志物。
