Olga Petrović, Petar Knezević, Jelena Marković, Srdjan Roncević
Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovica 5, 21000 Novi Sad, Serbia.
J Microbiol Methods. 2008 Aug;74(2-3):110-3. doi: 10.1016/j.mimet.2008.03.012. Epub 2008 Apr 11.
Toxic action of crude oil on the living world and ecosystems in general is a global problem of both aquatic and terrestrial environments. Bearing in mind the possibility of biodegradation of this toxicant, the procedures of determining counts and activity of cultivable microorganisms, and especially of bacteria responsible for degradation processes, are of great significance. The aim of this work was to study the possibility of modifying some solid media by adding triphenyltetrazolium chloride reagent as an indicator of the dehydrogenase activity, to develop a simple screening method for a simultaneous assessment of the count and activity of cultivable hydrocarbon-oxidizing bacteria in the oil-contaminated environments. The modified method appeared to be rapid and very suitable for the intended purposes.
原油对整个生物界和生态系统的毒性作用是水生和陆地环境的一个全球性问题。考虑到这种有毒物质的生物降解可能性，确定可培养微生物数量和活性的程序，尤其是负责降解过程的细菌的数量和活性的程序，具有重要意义。这项工作的目的是研究通过添加氯化三苯基四氮唑试剂作为脱氢酶活性指标来改良一些固体培养基的可能性，以开发一种简单的筛选方法，用于同时评估受油污染环境中可培养的烃氧化细菌的数量和活性。改良后的方法似乎快速且非常适合预期目的。
