血红素加氧酶-1的表达与孕鼠感染布鲁氏菌流产相关。

Expression of heme oxygenase-1 is associated with abortion caused by Brucella abortus infection in pregnant mice.

作者信息

Tachibana Masato, Watanabe Kenta, Yamasaki Yuki, Suzuki Hiroshi, Watarai Masahisa

机构信息

Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro-shi, Hokkaido 080-8555, Japan.

出版信息

Microb Pathog. 2008 Aug;45(2):105-9. doi: 10.1016/j.micpath.2008.04.002. Epub 2008 Apr 16.

DOI:10.1016/j.micpath.2008.04.002

PMID:18501554

Abstract

Brucella abortus is a facultative intracellular pathogen that can survive inside macrophages and trophoblast giant (TG) cells, and the causative agent of brucellosis. In the present study, we found that expression of heme oxygenase-1 (HO-1) in TG cells is correlated with abortion induced by B. abortus infection in pregnant mice. Expression of HO-1 in the placenta was decreased by B. abortus infection and treatment with cobalt-protoporphyrin (Co-PP), which is known to up-regulate HO-1 expression, inhibited abortion due to the bacterial infection. In TG cells, treatment with Co-PP was shown to up-regulate HO-1, whereas its expression was decreased by B. abortus infection. Such down-regulation of HO-1 in the TG cells was enhanced by IFN-gamma treatment. HO-1 down-regulation in TG cells due to knockdown or IFN-gamma treatment served to induce cell death caused by B. abortus infection. These results suggest that down-regulation of HO-1 in TG cells due to B. abortus infection is an important event in infectious abortion.

摘要

牛布鲁氏菌是一种兼性胞内病原体，可在巨噬细胞和滋养层巨细胞（TG细胞）内存活，是布鲁氏菌病的病原体。在本研究中，我们发现TG细胞中血红素加氧酶-1（HO-1）的表达与怀孕小鼠感染牛布鲁氏菌诱导的流产相关。牛布鲁氏菌感染可降低胎盘HO-1的表达，而用已知可上调HO-1表达的钴原卟啉（Co-PP）处理可抑制细菌感染导致的流产。在TG细胞中，Co-PP处理可上调HO-1表达，而牛布鲁氏菌感染则使其表达降低。γ-干扰素处理可增强TG细胞中HO-1的这种下调。由于敲低或γ-干扰素处理导致TG细胞中HO-1下调会引发牛布鲁氏菌感染所致的细胞死亡。这些结果表明，牛布鲁氏菌感染导致TG细胞中HO-1下调是感染性流产中的一个重要事件。