Anderson T D, Meador V P, Cheville N F
Vet Pathol. 1986 May;23(3):219-26. doi: 10.1177/030098588602300301.
Pregnant goats were given Brucella abortus intravenously or in uterine arteries, and tissues from the uterus and placentae were examined at various post-inoculation intervals to study mechanisms of placental infection. Placentitis was present by 5 days post-inoculation and abortions occurred within 11 days. B. abortus was identified in placentae by light microscopy and immunoperoxidase techniques. B. abortus was first seen in erythrophagocytic trophoblasts of the placentome. Subsequently, high numbers of B. abortus were seen in periplacentomal chorioallantoic trophoblasts. Trophoblast necrosis, chorioallantoic ulceration, and large numbers of B. abortus in chorionic villi were present in later stages of infection. These results suggest that entry and replication of B. abortus in trophoblasts precede placentome and fetal infection and that trophoblasts are the source of B. abortus for these tissues. Experimental caprine brucellosis closely resembles bovine and ovine brucellosis and it provides a model to study the intracellular development of B. abortus in trophoblasts.
给怀孕山羊静脉注射或经子宫动脉注射流产布鲁氏菌，在接种后的不同时间间隔检查子宫和胎盘组织，以研究胎盘感染的机制。接种后5天出现胎盘炎，11天内发生流产。通过光学显微镜和免疫过氧化物酶技术在胎盘中鉴定出流产布鲁氏菌。流产布鲁氏菌首先出现在胎盘小叶的吞噬红细胞的滋养层细胞中。随后，在胎盘周绒毛膜尿囊滋养层细胞中发现大量流产布鲁氏菌。感染后期出现滋养层细胞坏死、绒毛膜尿囊溃疡以及绒毛膜绒毛中有大量流产布鲁氏菌。这些结果表明，流产布鲁氏菌在滋养层细胞中的侵入和复制先于胎盘小叶和胎儿感染，并且滋养层细胞是这些组织中流产布鲁氏菌的来源。实验性山羊布鲁氏菌病与牛和绵羊布鲁氏菌病非常相似，它为研究流产布鲁氏菌在滋养层细胞中的细胞内发育提供了一个模型。
