Sonnen Andreas F-P, Rowe Arthur J, Andrew Peter W, Gilbert Robert J C
Division of Structural Biology, The Henry Wellcome Building for Human Genetics, University of Oxford, Oxford, UK.
Toxicon. 2008 Jun 15;51(8):1554-9. doi: 10.1016/j.toxicon.2008.03.032. Epub 2008 Apr 8.
Pneumolysin is a cytolytic toxin of Streptococcus pneumoniae, a causative agent of pneumonia and meningitis. The prepore and pore states of pneumolysin have recently been investigated by cryo-electron microscopy and atomic force microscopy, confirming the existence of arc-shaped as well as ring-form oligomers. Here we provide further insights into the pneumolysin oligomer by studying the interaction of pneumolysin with cholesterol crystals, comparing the results to those obtained for polyene antibiotics, which also bind cholesterol.
肺炎溶血素是肺炎链球菌的一种细胞溶解毒素，肺炎链球菌是肺炎和脑膜炎的病原体。最近通过冷冻电子显微镜和原子力显微镜研究了肺炎溶血素的前孔状态和孔状态，证实了弧形以及环形寡聚体的存在。在这里，我们通过研究肺炎溶血素与胆固醇晶体的相互作用，进一步深入了解肺炎溶血素寡聚体，并将结果与同样结合胆固醇的多烯抗生素的结果进行比较。
