Cafarchia Claudia, Gallo Sabrina, Danesi Patrizia, Capelli Gioia, Paradies Paola, Traversa Donato, Gasser Robin B, Otranto Domenico
Department of Veterinary Public Health, Faculty of Veterinary Medicine of Bari, Valenzano, Bari, Italy.
Acta Trop. 2008 Jul;107(1):25-9. doi: 10.1016/j.actatropica.2008.04.008. Epub 2008 Apr 15.
The relationship among the frequency, population size and phospholipase activity of Malasseziapachydermatis was investigated for dogs with Leishmania infantum infection (Li+) and those without evidence of this infection (Li-). A group of 188 dogs (141 without and 47 with skin lesions) was examined clinically, and samples were taken for the detection of Malassezia and L. infantum using various diagnostic methods. Malassezia was cultured from skin samples from 101 (53.7%) dogs and classified biochemically and molecularly as M. pachydermatis. A significantly higher mean population size of M. pachydermatis was cultured from the skin of L+ dogs compared with L- dogs. For M. pachydermatis, most phospolipase-producing cultures and the highest phospholipase activity were recorded for L- dogs with lesions and L+ dogs without lesions. The results showed that M. pachydermatis was a common commensal on dogs with or without L. infantum infection and established that L. infantum infection in dogs without skin lesions was associated with increased growth of M. pachydermatis and production of phospholipase in vitro.
对感染婴儿利什曼原虫（Li+）和未感染该寄生虫（Li-）的犬，研究了厚皮马拉色菌的频率、种群大小和磷脂酶活性之间的关系。对一组188只犬（141只无皮肤损伤，47只患有皮肤损伤）进行临床检查，并采集样本，使用各种诊断方法检测马拉色菌和婴儿利什曼原虫。从101只（53.7%）犬的皮肤样本中培养出马拉色菌，并通过生化和分子方法将其鉴定为厚皮马拉色菌。与Li-犬相比，从Li+犬皮肤培养出的厚皮马拉色菌平均种群大小显著更高。对于厚皮马拉色菌，在患有损伤的Li-犬和无损伤的Li+犬中，产生磷脂酶的培养物最多，磷脂酶活性最高。结果表明，厚皮马拉色菌是有无婴儿利什曼原虫感染犬的常见共生菌，并证实无皮肤损伤的犬感染婴儿利什曼原虫与体外厚皮马拉色菌生长增加和磷脂酶产生有关。
