Li Qiao-Yun, Niu Hong-Bin, Yin Jun, Wang Meng-Ben, Shao Hong-Bo, Deng De-Zhi, Chen Xiao-Xia, Ren Jiang-Ping, Li Yong-Chun
School of Life Science and Technology, Shanxi University, Taiyuan 030006, China.
Colloids Surf B Biointerfaces. 2008 Sep 1;65(2):220-5. doi: 10.1016/j.colsurfb.2008.04.007. Epub 2008 Apr 23.
To probe into the potential of relieving the oxidative damage of salt stress, we investigated the protective role of nitric oxide on barley under salt stress. Salt stress resulted in increased ion leakage, lipid peroxidation and protein oxidation in barley leaves. Simultaneous treatments of barley leaves with 50 microM sodium nitroprusside, a nitric oxide donor, alleviated the damage of salt stress, reflected by decreased ion leakage, and malendialdehyde (MDA), carbonyl, and hydrogen peroxide content in barley leaves. The presence of the nitric oxide donor increased the activities of superoxide dismutases (SOD), ascorbate peroxidases (APX), and catalases (CAT). Meantime, sodium nitroprusside addition increased accumulation of ferritin at the protein level, indicating that nitric oxide directly regulated ferritin accumulation. These results suggested that nitric oxide can effectively protect seedlings from salt stress damage by enhancing activities of antioxidant enzymes to quench the excessive reactive oxygen species caused by salt stress and inducing the increase of ferritin accumulation to chelate larger number of ferrous ion. Information from this study can be used to improve soil management practices for sustainable use of salt-affected soils in the future.
为探究缓解盐胁迫氧化损伤的潜力，我们研究了一氧化氮在盐胁迫下对大麦的保护作用。盐胁迫导致大麦叶片离子渗漏增加、脂质过氧化和蛋白质氧化。用一氧化氮供体50微摩尔硝普钠同时处理大麦叶片，减轻了盐胁迫的损伤，这表现为大麦叶片中离子渗漏、丙二醛（MDA）、羰基和过氧化氢含量降低。一氧化氮供体的存在增加了超氧化物歧化酶（SOD）、抗坏血酸过氧化物酶（APX）和过氧化氢酶（CAT）的活性。同时，添加硝普钠增加了蛋白质水平上铁蛋白的积累，表明一氧化氮直接调节铁蛋白的积累。这些结果表明，一氧化氮可通过增强抗氧化酶活性以淬灭盐胁迫引起的过量活性氧，并诱导铁蛋白积累增加以螯合更多亚铁离子，从而有效保护幼苗免受盐胁迫损伤。本研究所得信息可用于改进土壤管理措施，以便未来可持续利用盐渍土壤。
