Mrowietz C, Hiebl B, Franke R P, Park J-W, Jung F
Institute for Heart and Circulation Research, Hoyerswerda, Germany.
Clin Hemorheol Microcirc. 2008;39(1-4):281-6.
Various radiographic contrast media (RCM) significantly influence the morphology of erythrocytes, especially the formation of echinocytes [Scand. J. Clin. Lab. Invest. 35 (1975), 1-43; Microvasc. Res. 60 (2000), 193-200; Herz 23 (2003), 35-41]. Microscopic studies, however, have shown that these changes of erythrocyte morphology are possibly reversible [Acta Radiol. 37 (1996), 214-217]. The aim of this study was to proof if the RCM-induced echinocyte formation can be reversed by a resuspension in autologous plasma. In this study four RCMs were tested (Iodixanol, Iohexol, Iomeprol and Iopromide). These RCM induced echinocyte formation (after suspension of erythrocytes in plasma/RCM mixtures for 10 min at 37 degrees C), which was reversible after resuspension in autologous RCM-free plasma (resuspension time 5 min at 37 degrees C). Especially for Iomeprol and Iopromide - the RCMs which induced the strongest echinocyte formation - an echinocyte reduction from 94.2% to 44.5% and for Iopromide from 80.6% to 50.4% occurred. The echinocyte formation was influenced by the type of RCM as well as by the RCM concentration. The same was true for the reversibility of echinocyte formation due to resuspension in autologous plasma (type of RCM: p</=0.0001; concentration of RCM: p=0.0847). Iodixanol was associated with the least numbers echinocytes formed (after suspension in the plasma/RCM-mixture as well as after the resuspension in autologous plasma). A 100% reversibility back to discocytes was observed in none of the RCMs after resuspension in autologous RCM-free plasma.In conclusion, a significant reversibility of RCM-induced echinocyte formation in autologous plasma could be observed.
多种放射造影剂（RCM）会显著影响红细胞的形态，尤其是棘形红细胞的形成[《斯堪的纳维亚临床实验室研究杂志》35（1975），1 - 43；《微血管研究》60（2000），193 - 200；《心脏》23（2003），35 - 41]。然而，显微镜研究表明，这些红细胞形态的变化可能是可逆的[《放射学报》37（1996），214 - 217]。本研究的目的是验证将红细胞重新悬浮于自体血浆中是否能逆转RCM诱导的棘形红细胞形成。在本研究中，测试了四种RCM（碘克沙醇、碘海醇、碘美普尔和碘普罗胺）。这些RCM诱导了棘形红细胞形成（红细胞在血浆/RCM混合物中于37℃悬浮10分钟后），在重新悬浮于无RCM的自体血浆中（于37℃重新悬浮5分钟）后是可逆的。特别是对于碘美普尔和碘普罗胺——诱导最强棘形红细胞形成的RCM——棘形红细胞比例从94.2%降至44.5%，碘普罗胺则从80.6%降至50.4%。棘形红细胞的形成受RCM类型以及RCM浓度的影响。因重新悬浮于自体血浆导致的棘形红细胞形成的可逆性情况也是如此（RCM类型：p≤0.0001；RCM浓度：p = 0.0847）。碘克沙醇形成的棘形红细胞数量最少（在悬浮于血浆/RCM混合物后以及重新悬浮于自体血浆后）。在重新悬浮于无RCM的自体血浆后，没有一种RCM观察到能100%逆转为双凹圆盘状红细胞。总之，在自体血浆中可观察到RCM诱导的棘形红细胞形成具有显著的可逆性。
