拟茎点霉属真菌的三种新型抗菌代谢产物

Three new antimicrobial metabolites of Phomopsis sp.

作者信息

Du Xiping, Lu Chunhua, Li Yaoyao, Zheng Zhonghui, Su Wenjin, Shen Yuemao

机构信息

Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, Xiamen University, China.

出版信息

J Antibiot (Tokyo). 2008 Apr;61(4):250-3. doi: 10.1038/ja.2008.37.

DOI:10.1038/ja.2008.37

PMID:18503206

Abstract

Two new 10-membered macrolides, named Phomolides A (1) and B (2), and one new derivative of benzofuran (3) were isolated from the culture of Phomopsis sp. hzla01-1, and their structures were elucidated based on spectroscopic analyses, particularly 2D NMR experiments. Compounds 1, 2 and 3 showed significant antimicrobial activities against Escherichia coli CMCC44103, Candida albicans AS2.538, Saccharomyces cerevisiae ATCC9763 with MICs 5~10 mug/ml. The most significant, compound 3 showed strong activity against Bacillus subtilis CMCC63501 with the MIC value 1.25 microg/ml. None of the three compounds showed significant cytotoxicity against the HeLa cell line at 10 microg/ml.

摘要

从拟茎点霉属菌株hzla01 - 1的培养物中分离出两种新的十元大环内酯类化合物，命名为茎点霉醇A（1）和茎点霉醇B（2），以及一种新的苯并呋喃衍生物（3），并通过光谱分析，特别是二维核磁共振实验确定了它们的结构。化合物1、2和3对大肠杆菌CMCC44103、白色念珠菌AS2.538、酿酒酵母ATCC9763显示出显著的抗菌活性，最低抑菌浓度（MIC）为5~10μg/ml。最显著的是，化合物3对枯草芽孢杆菌CMCC63501显示出强活性，MIC值为1.25μg/ml。这三种化合物在10μg/ml时对HeLa细胞系均未显示出显著的细胞毒性。