Gagey Nathalie, Emond Matthieu, Neveu Pierre, Benbrahim Chouaha, Goetz Bernard, Aujard Isabelle, Baudin Jean-Bernard, Jullien Ludovic
Département de Chimie and Laboratoire de Physique Statistique, Ecole Normale Supérieure, Paris Cedex 05, France.
Org Lett. 2008 Jun 19;10(12):2341-4. doi: 10.1021/ol800284g. Epub 2008 May 27.
This paper evaluates a series of photolabile protecting groups with built-in fluorescence reporting. They rely on readily available o-acetoxyphenyl methyloxazolones as activated precursors. Alcohol substrates are easily caged. The resulting photoactivable esters exhibit large one- and two-photon uncaging cross sections. The alcohol substrates are quantitatively released in a 1:1 molar ratio with a strongly fluorescent coumarin coproduct that serves as a reporter to quantify substrate delivery.
本文评估了一系列具有内置荧光报告功能的光不稳定保护基团。它们依赖于易于获得的邻乙酰氧基苯基甲基恶唑啉作为活化前体。醇底物易于被笼蔽。所得的可光活化酯表现出较大的单光子和双光子解笼截面。醇底物以1:1的摩尔比定量释放，同时产生一种强荧光香豆素副产物作为报告物来定量底物传递。
