Lee Hsien-Ming, Larson Daniel R, Lawrence David S
Department of Chemistry, The University of North Carolina, Chapel Hill, North Carolina 27599-3290, USA.
ACS Chem Biol. 2009 Jun 19;4(6):409-27. doi: 10.1021/cb900036s.
Biological systems are characterized by a level of spatial and temporal organization that often lies beyond the grasp of present day methods. Light-modulated bioreagents, including analogs of low molecular weight compounds, peptides, proteins, and nucleic acids, represent a compelling strategy to probe, perturb, or sample biological phenomena with the requisite control to address many of these organizational complexities. Although this technology has created considerable excitement in the chemical community, its application to biological questions has been relatively limited. We describe the challenges associated with the design, synthesis, and use of light-responsive bioreagents; the scope and limitations associated with the instrumentation required for their application; and recent chemical and biological advances in this field.
生物系统的特征在于其空间和时间组织水平，这常常超出了当今方法的理解范围。光调制生物试剂，包括低分子量化合物、肽、蛋白质和核酸的类似物，是一种极具吸引力的策略，可通过必要的控制来探测、扰动或采样生物现象，以解决其中许多组织复杂性问题。尽管这项技术在化学界引起了相当大的轰动，但其在生物学问题上的应用相对有限。我们描述了与光响应生物试剂的设计、合成和使用相关的挑战；其应用所需仪器的范围和局限性；以及该领域最近的化学和生物学进展。
