Gerasimov A M, Toporova S M, Furtseva L N, Berezhnoy A P, Vilensky E V, Alekseeva R I
Department of Biochemistry, Central Institute of Traumatology and Orthopaedics, Moscow, USSR.
Clin Orthop Relat Res. 1991 May(266):53-63.
Unicameral bone cyst fluid possesses N-acetyl-beta-D-glucosaminidase, beta-glucuronidase, PZ-peptidase, cathepsin D, acid phosphatase, N-acetyl-beta-D galactosaminidase, and beta-galactosidase activities. The activities of lysosomal enzymes in the cyst fluid are, as a rule, higher than in the serum, whereas the total protein content is lower. The content of collagen degradation products in the cyst fluid is higher compared to the serum. In bone cavity wall tissues, the collagen content is decreased. Adenosine 3':5'-cyclic phosphate and cyclic guanosine 3,5'-monophosphate accumulate in the cyst cavity. However, in some cases, there is no correlation among the activities of lysosomal enzymes in the cyst fluid, blood serum, and cyst wall tissues. The ratios of lysosomal enzyme activities in the cyst fluid differ from those in the cyst wall tissues, cultured skin fibroblasts, and blood polymorphonuclear leucocytes. The lack of coincidence of enzymatic spectra of the cyst fluid, wall tissues, and serum is suggestive of the diversity of ways of lysosomal enzyme enter the cyst cavity, i.e., blood, cyst fluid cells, and cyst cavity walls. The cysts with different locations (i.e., active and latent cysts) have similar lysosomal lytic potentials. The presence in the cyst cavity of extracellular lysosomal enzymes and collagen degradation products testifies to the permanent corrosion of the cyst cavity walls from the inside as well as to the increase in the osmotic pressure of the cyst fluid. Lysosome destruction should be regarded as an important pathogenetic factor that requires surgical or pharmacologic correction or both in the course of bone cyst management.
单房性骨囊肿液具有N - 乙酰 - β - D - 氨基葡萄糖苷酶、β - 葡萄糖醛酸酶、PZ - 肽酶、组织蛋白酶D、酸性磷酸酶、N - 乙酰 - β - D - 半乳糖胺酶和β - 半乳糖苷酶活性。通常，囊肿液中溶酶体酶的活性高于血清，而总蛋白含量则较低。与血清相比，囊肿液中胶原降解产物的含量更高。在骨腔壁组织中，胶原含量降低。腺苷3':5'-环磷酸和环鸟苷3,5'-单磷酸在囊肿腔内积聚。然而，在某些情况下，囊肿液、血清和囊肿壁组织中溶酶体酶的活性之间没有相关性。囊肿液中溶酶体酶活性的比率与囊肿壁组织、培养的皮肤成纤维细胞和血液多形核白细胞中的比率不同。囊肿液、壁组织和血清的酶谱缺乏一致性，提示溶酶体酶进入囊肿腔的方式具有多样性，即通过血液、囊肿液细胞和囊肿腔壁。不同部位的囊肿（即活动期和静止期囊肿）具有相似的溶酶体溶解潜能。囊肿腔内存在细胞外溶酶体酶和胶原降解产物，证明囊肿腔壁从内部持续受到侵蚀，也证明囊肿液渗透压升高。溶酶体破坏应被视为骨囊肿治疗过程中需要手术或药物纠正或两者兼有的重要致病因素。
