Boulaflous Aurelia, Faso Carmen, Brandizzi Federica
MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA.
Traffic. 2008 Sep;9(10):1613-7. doi: 10.1111/j.1600-0854.2008.00769.x. Epub 2008 May 22.
The Golgi apparatus is a vital organelle in eukaryotic cells. It grabs and processes secretory materials synthesized by the endoplasmic reticulum (ER) before sorting them to their destination. The Golgi also receives materials from vacuoles/lysosomes and the plasma membrane for further recycling to other compartments within the cell (1) (Figure 1). Given the vital role of the Golgi in a cell, it is important to understand how this organelle attains and maintains its structural and functional integrity during the intense processes of membrane traffic. Despite an equally central role of the Golgi in membrane traffic in eukaryotes, the organization of this organelle has some unique features in each cell system. Therefore, the wealth of information available on the structure and activity of the Golgi in one system is not always directly transferable to others. However, certain morphological and functional aspects are common among cell systems. Therefore, studying the factors that regulate organelle biogenesis and organization of the Golgi apparatus is important in basic cell biology of eukaryotes and may also contribute to a better understanding of how different cell systems have evolved. In this study, we report on the identification of Golgi mutants in plant cells. We have developed a screen that is a promising strategy not only for the identification of genes responsible for the morphological and functional integrity of the plant Golgi but could also provide fundamental information on other multicellular systems for which the power of forward genetics cannot be exploited as easily as in Arabidopsis.
高尔基体是真核细胞中的一种重要细胞器。它获取并处理由内质网（ER）合成的分泌物质，然后将它们分类运输到目的地。高尔基体还接收来自液泡/溶酶体和质膜的物质，以便进一步循环利用到细胞内的其他区室（1）（图1）。鉴于高尔基体在细胞中的重要作用，了解该细胞器在激烈的膜运输过程中如何获得并维持其结构和功能完整性非常重要。尽管高尔基体在真核生物的膜运输中同样起着核心作用，但在每个细胞系统中，这个细胞器的组织方式都有一些独特之处。因此，在一个系统中获得的关于高尔基体结构和活性的丰富信息并不总是能直接应用于其他系统。然而，某些形态和功能方面在细胞系统中是共有的。因此，研究调节高尔基体细胞器生物发生和组织的因素在真核生物的基础细胞生物学中很重要，也可能有助于更好地理解不同细胞系统是如何进化的。在这项研究中，我们报告了在植物细胞中鉴定高尔基体突变体的情况。我们开发了一种筛选方法，这不仅是一种很有前景的策略，可用于鉴定负责植物高尔基体形态和功能完整性的基因，还能为其他多细胞系统提供基础信息，而对于这些系统，正向遗传学的力量不像在拟南芥中那样容易被利用。
