Karataş Hüseyin, Ağaoğlu Y Sabit
Department of Horticulture, Faculty of Agriculture, Dicle University, Diyarbakir, Turkey.
Hereditas. 2008 Apr;145(2):58-63. doi: 10.1111/j.0018-0661.2008.02011.x.
To estimate genetic relationships among 46 local grape cultivars, RAPD analysis was performed with 25 decamer primers selected from a total of 60 primers. Genetic relationships among these cultivars were determined by calculating similarity indexes, from which a dendogram was derived. There was high genetic variation among the cultivars, with values of genetic diversity ranging from 0.553 to 0.952 using the Jaccard coefficient. UPGMA analysis of a distance matrix produced a dendogram with six clusters. The relatively high genetic similarity ratios observed for the cultivars was also reflected in the dendogram. In general, no relationship was encountered between the genetic similarity ratios of the cultivars and the results of previous ampelographic analyses.
为了评估46个本地葡萄品种之间的亲缘关系，从总共60个引物中挑选出25个十聚体引物进行随机扩增多态性DNA（RAPD）分析。通过计算相似性指数来确定这些品种之间的亲缘关系，并据此绘制出聚类图。这些品种间存在高度的遗传变异，使用杰卡德系数计算得出的遗传多样性值在0.553至0.952之间。对距离矩阵进行非加权组平均法（UPGMA）分析得到了一个包含六个聚类的聚类图。品种间相对较高的遗传相似率在聚类图中也有所体现。总体而言，品种的遗传相似率与先前葡萄品种形态学分析的结果之间没有关联。
