利用RAPD标记评估球茎大麦（Hordeum bulbosum L.）的遗传和地理变异。

Genetic and geographic variation of bulbous barley (Hordeum bulbosum L.) assessed by rapd markers.

作者信息

Okumus A, Uzun F

机构信息

Biometry-Genetics Unit, Animal Science Department, Agriculture Faculty, Ondokuz Mayis University, Samsun 55139, Turkey.

出版信息

Genetika. 2007 Mar;43(3):377-81.

PMID:17486757

Abstract

Estimated the genetic relationships among 21 barley accessions from 17 bulbous barley (H. bulbosum L.), 4 cultivated barley (H. vulgare L.) collected from different part of Turkey were investigated using Random Amplified Polymorphic DNA (RAPD). Eleven informative primers amplified 111 markers of which 98 (89.8%) were polymorphic. A dendogram was constructed using the UPGMA method based on the RAPD markers. The range of genetic similarity was from 0.111 to 0.815. The accessions were grouped into two main clusters based on the molecular data. The H. vulgare and H. bulbosum separated into two groups in the Principle Component Analysis.

摘要

利用随机扩增多态性DNA（RAPD）技术，对从土耳其不同地区收集的17份球茎大麦（H. bulbosum L.）和4份栽培大麦（H. vulgare L.）共21份大麦种质资源的遗传关系进行了研究。11条信息引物扩增出111个标记，其中98个（89.8%）为多态性标记。基于RAPD标记，采用非加权组平均法（UPGMA）构建了聚类图。遗传相似性范围为0.111至0.815。根据分子数据，这些种质资源被分为两个主要类群。在主成分分析中，H. vulgare和H. bulbosum被分为两组。