Watanabe Naoto, Horie Syunji, Spina Domenico, Michael Gregory J, Page Clive P, Priestley John V
Department of Respiratory and Infectious Diseases, St. Marianna University School of Medicine, Kawasaki, Japan.
Int Arch Allergy Immunol. 2008;146 Suppl 1:28-32. doi: 10.1159/000126057. Epub 2008 May 27.
We previously found many transient receptor potential vanilloid receptor subtype 1 (TRPV1) axons in the tracheal smooth muscle and epithelium of the guinea pig airway. One report indicates that the number of TRPV1 axons is significantly increased in patients with cough variant asthma.
To determine whether the distribution of TRPV1 in the airways is altered in guinea pigs with an allergic phenotype.
Ten guinea pigs were assigned to 2 groups in a double-blind study. Five animals were sensitized with ovalbumin and the other 5 underwent sham sensitization. Cryopreserved sections (30 microm) of tracheal tissues removed from each animal were stained with polyclonal serum rabbit anti-TRPV1 antibody (1:30,000) and examined by confocal microscopy.
Axons immunoreactive to TRPV1 localized to fine axons within the epithelium and around areas of smooth muscle, were more densely stained and frequent in the ovalbumin than in the sham group.
The number of TRPV1-immunoreactive axons in the trachea increases under allergic inflammatory conditions.
我们之前在豚鼠气道的气管平滑肌和上皮中发现了许多瞬时受体电位香草酸受体1型（TRPV1）轴突。一份报告指出，咳嗽变异性哮喘患者的TRPV1轴突数量显著增加。
确定具有过敏表型的豚鼠气道中TRPV1的分布是否发生改变。
在一项双盲研究中，将10只豚鼠分为2组。5只动物用卵清蛋白致敏，另外5只进行假致敏。从每只动物身上取出的气管组织冷冻切片（30微米）用兔抗TRPV1多克隆血清抗体（1:30,000）染色，并用共聚焦显微镜检查。
对TRPV1免疫反应的轴突定位于上皮内和平滑肌周围的细轴突，在卵清蛋白组中染色更密集且更常见，比假致敏组更多。
在过敏性炎症条件下，气管中TRPV1免疫反应性轴突的数量增加。
