Kawakami Ayako, Koketsu Rikiya, Suzukawa Maho, Nagao Mizuho, Hiraguchi Yukiko, Tokuda Reiko, Fujisawa Takao, Nagase Hiroyuki, Ohta Ken, Yamamoto Kazuhiko, Yamaguchi Masao
Department of Allergy and Rheumatology, University of Tokyo Graduate School of Medicine, Tokyo, Japan.
Int Arch Allergy Immunol. 2008;146 Suppl 1:54-60. doi: 10.1159/000126062. Epub 2008 May 27.
Japanese cedar pollen is by far the most important cause of allergic rhinitis in Japan. In this study, we assessed the induction of blocking antibody during specific immunotherapy (SIT) using a recently standardized allergen extract from Japanese cedar pollen.
Basophils from nonallergic subjects were passively sensitized with serum samples prepared from pollinosis patients before and after SIT; all patients showed good clinical efficacy. The cells were then stimulated with the standardized allergen, and histamine release was measured. In most experiments, the basophil stimulation buffer contained 1% serum.
Pollinosis patients' sera obtained both before and after SIT showed essentially similar sensitizing capacity for basophils. Basophil degranulation in response to a relatively low concentration of pollen extract was effectively suppressed by addition of post-SIT serum samples, indicating the presence of blocking antibody. The blocking antibody was IgG, and its potency varied widely among the donor patients.
The standardized allergen extract from Japanese cedar pollen is useful not only for clinical application in SIT, but also for testing for induction of blocking antibody during SIT.
在日本，日本雪松花粉是过敏性鼻炎的最主要病因。在本研究中，我们使用一种最近标准化的日本雪松花粉变应原提取物，评估了特异性免疫疗法（SIT）期间封闭抗体的诱导情况。
用花粉症患者在SIT前后制备的血清样本对非过敏受试者的嗜碱性粒细胞进行被动致敏；所有患者均显示出良好的临床疗效。然后用标准化变应原刺激细胞，并测量组胺释放。在大多数实验中，嗜碱性粒细胞刺激缓冲液含有1%血清。
SIT前后获得的花粉症患者血清对嗜碱性粒细胞的致敏能力基本相似。添加SIT后血清样本可有效抑制嗜碱性粒细胞对相对低浓度花粉提取物的脱颗粒反应，表明存在封闭抗体。该封闭抗体为IgG，其效力在供体患者中差异很大。
标准化的日本雪松花粉变应原提取物不仅可用于SIT的临床应用，还可用于检测SIT期间封闭抗体的诱导情况。
