Chen Hong, Wang Liang, Zhang Yanxia, Li Dan, McClung W Glenn, Brook Michael A, Sheardown Heather, Brash John L
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, and School of Materials Science and Engineering, Wuhan University of Technology, Wuhan 122 Luoshi Rd. 430070, China.
Macromol Biosci. 2008 Sep 9;8(9):863-70. doi: 10.1002/mabi.200800014.
PDMS surfaces have been modified to confer both resistance to non-specific protein adsorption and clot lyzing properties. The properties and chemical compositions of the surfaces have been investigated using water contact angle measurements, ATR FT-IR spectroscopy, and XPS. The ability of the PEG component to suppress non-specific protein adsorption was assessed by measurement of radiolabeled fibrinogen uptake from buffer. The adsorption of plasminogen from human plasma to the various surfaces was studied. In vitro experiments demonstrated that lysine-immobilized surfaces with free epsilon-amino groups were able to dissolve fibrin clots, following exposure to plasma and tissue plasminogen activator. [Figure: see text].
聚二甲基硅氧烷（PDMS）表面已被改性，以兼具抗非特异性蛋白质吸附和凝块溶解特性。已使用水接触角测量、衰减全反射傅里叶变换红外光谱（ATR FT-IR）和X射线光电子能谱（XPS）研究了这些表面的特性和化学成分。通过测量缓冲液中放射性标记纤维蛋白原的摄取量，评估了聚乙二醇（PEG）成分抑制非特异性蛋白质吸附的能力。研究了人血浆中纤溶酶原在各种表面上的吸附情况。体外实验表明，具有游离ε-氨基的赖氨酸固定化表面在暴露于血浆和组织纤溶酶原激活剂后能够溶解纤维蛋白凝块。[图：见正文]
