Dimitrova Anelia A, Strashimirov Denko S, Russeva Adelaida L, Betova Tatyana M, Tzachev Kamen N
Department of General Biology and Pathophysiology, Medical University, Pleven, Bulgaria.
Folia Med (Plovdiv). 2007;49(3-4):52-7.
The aim of the present study was to investigate the effect of zinc on the activity of copper-zinc superoxide dismutase (Cu/Zn SOD), the lipid profile, the arterial blood pressure and the morphological changes of the aorta in male spontaneously hypertensive rats (SHR).
The study included 52 male SHR animals. The animals were randomly asigned into three groups to be fed one of three zink diets--in group one animals (G1, control group) the zinc content was 50 mg/kg, in group two (G2)--155 mg/kg and in group three (G3) 250 mg/kg. The laboratory chow zinc content and the zinc and copper serum levels were measured by a direct flame atomic-absorption spectrophotometry. The Cu/Zn SOD activity was measured in the erythrocytes using a reagent from RANSOD (RANDOX Laboratories). Systolic arterial blood pressure was measured on the rat's tail (tail cuff) by an indirect method without anesthesia (Ugo Basile). The lipid profile was determined with ABX reagents (France). Histological investigations of the aortas were performed using histochemical and electron microscopic methods.
Statistically significant increase of Cu/Zn SOD activity (p < 0.05) was found in G2 (2276.88 +/- 145.53) and G3 (2337.31 +/- 146.25) in comparison with the control group of animals (2099.46 +/- 111.73). There was significant decrease of serum LDL levels (p < 0.05) in G3 (1.09 +/- 0.32) compared with G2 (1.19 +/- 0.42) and G1 (1.62 +/- 0.28). Increased number of migrated smooth muscle cells was found in G1 animals. Lipid drops were increased in the aortic wall of SHR animals and then decreased after zinc diet was applied.
The results indicate that high zinc content in the diet has a protective effect as it increases Cu/Zn SOD activity, decreases the LDL serum levels and the lipid infiltration of the aorta in male SHR animals.
本研究旨在探讨锌对雄性自发性高血压大鼠（SHR）铜锌超氧化物歧化酶（Cu/Zn SOD）活性、血脂谱、动脉血压及主动脉形态学变化的影响。
本研究纳入52只雄性SHR动物。将动物随机分为三组，分别给予三种锌含量不同的饮食——第一组动物（G1，对照组）锌含量为50 mg/kg，第二组（G2）为155 mg/kg，第三组（G3）为250 mg/kg。采用直接火焰原子吸收分光光度法测定实验室饲料中的锌含量以及血清锌和铜水平。使用兰多克斯实验室（RANDOX Laboratories）的试剂测定红细胞中的Cu/Zn SOD活性。采用间接方法（乌戈·巴齐勒公司Ugo Basile）在未麻醉的大鼠尾部（尾套法）测量收缩期动脉血压。使用法国ABX试剂测定血脂谱。采用组织化学和电子显微镜方法对主动脉进行组织学研究。
与动物对照组（2099.46±111.73）相比，G2组（2276.88±145.53）和G3组（2337.31±146.25）的Cu/Zn SOD活性有统计学意义的升高（p<0.05）。与G2组（1.19±0.42）和G1组（1.62±0.28）相比，G3组（1.09±0.32）的血清低密度脂蛋白（LDL）水平显著降低（p<0.05）。在G1组动物中发现迁移的平滑肌细胞数量增加。SHR动物主动脉壁中的脂滴增加，在给予锌饮食后减少。
结果表明，饮食中高锌含量具有保护作用，因为它可提高雄性SHR动物的Cu/Zn SOD活性，降低血清LDL水平以及主动脉的脂质浸润。
