Iskra M, Majewski W
Department of General Chemistry, Karol Marcinkowski University of Medical Sciences, 6 Grunwaldzka Street, 60-780 PoznaA, Poland.
Int J Clin Lab Res. 1999;29(2):64-7. doi: 10.1007/s005990050065.
Cu,Zn-superoxide dismutase is an endogenous scavanger of superoxide radicals (O(2)(*-)) which also induce the synthesis of this enzyme. Ceruloplasmin is an antioxidant and acute-phase reactant. Changes in the synthesis of both enzymes are related to the metabolism of copper and zinc. Concentrations of copper and zinc were previously found to be increased in the serum and arterial wall of atherosclerotic subjects. The aim of this study was to investigate the Cu,Zn-superoxide dismutase activity in erythrocytes and ceruloplasmin activity in serum, and to measure serum concentrations of copper, zinc, and malonyldialdehyde in patients with moderate and critical chronic ischemia of the lower limbs. A group of 26 patients with chronic arterial occlusion of the lower limbs was divided into two groups depending on the degree of ischemia: moderate and critical. Cu,Zn-superoxide dismutase activity in erythrocytes was measured using the RANSOD kit, the serum ceruloplasmin oxidase activity was determined with o-dianisidine as a substrate. Copper and zinc concentrations in serum were determined by atomic absorption spectrometry. There was an increase in the ceruloplasmin activity and serum copper concentration in critical ischemia (194.4+/-51.94 U/l and 23.5+/-4.2 micromol/l, respectively) compared with moderate ischemia (139.1+/-34.9 U/l and 18.5+/-2.0 micromol/l, respectively). The Cu,Zn-superoxide dismutase activity in erythrocytes was higher in moderate ischemia (2,657+/-1,564 U/g hemoglobin) than in controls (1,205+/- 353 U/g hemoglobin), but not different from critical ischemia. There was a negative correlation for Cu,Zn-superoxide dismutase and ceruloplasmin (r=-0.60, P</=0.05) in critical ischemia.
铜锌超氧化物歧化酶是超氧阴离子自由基（O₂⁻）的内源性清除剂，超氧阴离子自由基也可诱导该酶的合成。铜蓝蛋白是一种抗氧化剂和急性期反应物。这两种酶合成的变化都与铜和锌的代谢有关。此前发现，动脉粥样硬化患者血清和动脉壁中的铜和锌浓度会升高。本研究的目的是调查下肢中度和重度慢性缺血患者红细胞中的铜锌超氧化物歧化酶活性以及血清中的铜蓝蛋白活性，并测量血清中铜、锌和丙二醛的浓度。一组26例下肢慢性动脉闭塞患者根据缺血程度分为两组：中度和重度。使用RANSOD试剂盒测量红细胞中的铜锌超氧化物歧化酶活性，以邻联茴香胺为底物测定血清铜蓝蛋白氧化酶活性。采用原子吸收光谱法测定血清中的铜和锌浓度。与中度缺血（分别为139.1±34.9 U/l和18.5±2.0 μmol/l）相比，重度缺血时铜蓝蛋白活性和血清铜浓度升高（分别为194.4±51.94 U/l和23.5±4.2 μmol/l）。中度缺血时红细胞中的铜锌超氧化物歧化酶活性（2657±1564 U/g血红蛋白）高于对照组（1205±353 U/g血红蛋白），但与重度缺血无差异。在重度缺血时，铜锌超氧化物歧化酶和铜蓝蛋白呈负相关（r = -0.60，P≤0.05）。
