Bowie D, Smart T G
Department of Pharmacology, School of Pharmacy, London, U.K.
Neurosci Lett. 1991 Jan 2;121(1-2):68-72. doi: 10.1016/0304-3940(91)90651-9.
Excitatory amino acid (EAA) receptors were studied in Xenopus laevis oocytes microinjected with 1-day-old chick brain mRNA using a two-electrode voltage clamp technique. EAA agonists could be differentially characterised according to their current/voltage (I/V) relationships; I/V plots to kainate (KA) and domoate (Dom) inwardly rectified at positive membrane potentials whereas quisqualate (QA), alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and L-glutamate (L-Glu) produced linear I/V plots. Interestingly, the non-NMDA (N-methyl-D-aspartate) agonist, 5-bromowillardiine (BrW), displayed properties similar to KA and Dom. It is proposed that BrW acts as a KA-like agonist on chick EAA receptors expressed in Xenopus oocytes.
使用双电极电压钳技术，在显微注射1日龄鸡脑信使核糖核酸（mRNA）的非洲爪蟾卵母细胞中研究兴奋性氨基酸（EAA）受体。EAA激动剂可根据其电流/电压（I/V）关系进行差异表征；对海人藻酸（KA）和软骨藻酸（Dom）的I/V图在正膜电位时向内整流，而quisqualate（QA）、α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）和L-谷氨酸（L-Glu）产生线性I/V图。有趣的是，非N-甲基-D-天冬氨酸（NMDA）激动剂5-溴威尔德菌素（BrW）表现出与KA和Dom相似的特性。有人提出，BrW在非洲爪蟾卵母细胞中表达的鸡EAA受体上作为一种类似KA的激动剂起作用。
