Rassendren F A, Lory P, Pin J P, Bockaert J, Nargeot J
CNRS Centre de Recherche de Biochimie Macromoléculaire, INSERM U 249, Montpellier, France.
Neurosci Lett. 1989 May 8;99(3):333-9. doi: 10.1016/0304-3940(89)90469-2.
Electrophysiological recording was used to study non-N-methyl-D-aspartate (NMDA) excitatory amino acid (EAA) receptors after injection of rat brain ribonucleic acid (RNA) in Xenopus laevis oocytes. Quisqualate (QA) induced two types of responses, a smooth one and an oscillatory one. These responses are probably mediated by the ionotropic (QAi, a cationic channel) and the metabotropic (QAp, a newly discovered receptor coupled to phospholipase C) QA receptors respectively. alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) only induced a smooth inward current suggesting that it acts only on QAi. Kainate (KA) also induced a smooth inward current, the maximal KA response being 10-fold higher than the maximal AMPA. AMPA inhibited the KA response in a dose-dependent and competitive manner. Amongst various complex hypotheses the simplest to explain these results would be that KA and AMPA both activate the same receptor-channel complex, AMPA inducing a smaller response than KA.
采用电生理记录法，研究在非洲爪蟾卵母细胞中注射大鼠脑核糖核酸（RNA）后非N-甲基-D-天冬氨酸（NMDA）兴奋性氨基酸（EAA）受体的情况。quisqualate（QA）诱导出两种反应，一种是平稳反应，另一种是振荡反应。这些反应可能分别由离子型（QAi，一种阳离子通道）和代谢型（QAp，一种新发现的与磷脂酶C偶联的受体）QA受体介导。α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）仅诱导出平稳的内向电流，表明它仅作用于QAi。海人酸（KA）也诱导出平稳的内向电流，KA的最大反应比AMPA的最大反应高10倍。AMPA以剂量依赖性和竞争性方式抑制KA反应。在各种复杂的假设中，解释这些结果最简单的假设是KA和AMPA都激活相同的受体-通道复合物，AMPA诱导的反应比KA小。
