Cetin E, Cengiz B, Gunduz E, Gunduz M, Nagatsuka H, Bekir-Beder L, Fukushima K, Pehlivan D, N M Ozaslan, Nishizaki K, Shimizu K, Nagai N
Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
Neoplasma. 2008;55(4):299-304.
Head and neck squamous cell carcinoma (HNSCC) is a diverse group of cancers that are frequently aggressive in their biologic behavior. Inactivation of tumor suppressor gene (TSG) is one of the most critical steps leading to HNSCC. Loss of heterozygosity analysis is very sensitive method for the detection of frequent allelic loss in a chromosomal locus. This method has been considered as an important evidence for the localization of TSGs. We analyzed loss of heterozygosity (LOH) at chromosome 4q22-35 region by using 14 polymorphic microsatellite markers in 83 matched normal and HNSCC tissues. LOH was detected at least in one location in 71 of 83 (86%) tumor tissues. Frequent deletions were detected at the location of microsatellite markers, D4S2909 (46%), D4S2623 (51%), D4S406 (48%), D4S1644 (45%) and D4S2979 (40%). Four different frequently deleted regions at 4q22, 4q25, 4q31 and 4q34-35 were observed. These regions include several putative TSGs such as Caspase-6, SMARCAD1, SMARCA5, SAP30 and ING2. Further molecular analysis of each gene should be performed to clarify their roles in head and neck squamous cell carcinogenesis.
头颈部鳞状细胞癌（HNSCC）是一组多样的癌症，其生物学行为通常具有侵袭性。肿瘤抑制基因（TSG）的失活是导致HNSCC的最关键步骤之一。杂合性缺失分析是检测染色体位点频繁等位基因缺失的非常灵敏的方法。该方法被认为是TSG定位的重要证据。我们使用14个多态性微卫星标记，对83对匹配的正常组织和HNSCC组织进行了4q22 - 35区域杂合性缺失（LOH）分析。在83个肿瘤组织中的71个（86%）至少在一个位点检测到了LOH。在微卫星标记D4S2909（46%）、D4S2623（51%）、D4S406（48%）、D4S1644（45%）和D4S2979（40%）位点检测到频繁缺失。观察到4q22、4q25、4q31和4q34 - 35有四个不同的频繁缺失区域。这些区域包括几个假定的TSG，如半胱天冬酶 - 6、SMARCAD1、SMARCA5、SAP30和ING2。应进一步对每个基因进行分子分析，以阐明它们在头颈部鳞状细胞癌发生中的作用。
